2026-04-14T13:26:45Zhttps://uvadoc.uva.es/oai/requestoai:uvadoc.uva.es:10324/714712024-12-11T10:18:29Zcom_10324_1138com_10324_931com_10324_894col_10324_1226
Usategui Martín, Ricardo
Puertas Neyra, Kevin Louis
García Gutiérrez, María Teresa
Fuentes, Manuel
Pastor Jimeno, José Carlos
Fernández Bueno, Iván
2024-11-14T13:37:30Z
2024-11-14T13:37:30Z
2020
Molecular Therapy - Methods & Clinical Development 2020;17:1155-1166
2329-0501
https://uvadoc.uva.es/handle/10324/71471
10.1016/j.omtm.2020.05.003
1155
1166
Molecular Therapy - Methods & Clinical Development
17
Retinal photoreceptor degeneration occurs frequently in
several neurodegenerative retinal diseases such as age-related
macular degeneration, retinitis pigmentosa, or genetic retinal
diseases related to the photoreceptors. Despite the impact on
daily life and the social and economic consequences, there is
no cure for these diseases. Considering this, cell-based therapy
may be an optimal therapeutic option. This study evaluated the
neuroprotective in vitro potential of a secretome of human
bone marrow mesenchymal stem cells (MSCs) for retinal pho-
toreceptors in vitro. We analyzed the photoreceptor morpho-
logic changes and the paracrine factors secreted by human
bone marrow MSCs in a physically separated co-culture with
degenerated neuroretinas, using organotypic neuroretinal
cultures. The results showed that the secretome of human
bone marrow MSCs had a neuroprotective effect over the
neuroretinal general organization and neuropreserved the
photoreceptors from degeneration probably by secretion of
neuroprotective proteins. The study of the expression of
1,000 proteins showed increased paracrine factors secreted by
MSCs that could be crucial in the neuroprotective effect of
the stem cell secretome over in vitro retinal degeneration.
The current results reinforce the hypothesis that the paracrine
effect of the human bone marrow MSCs may slow photore-
ceptor neurodegeneration and be a therapeutic option in
retinal photoreceptor degenerative diseases.
eng
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Human Mesenchymal Stem Cell Secretome Exhibits a Neuroprotective Effect over In Vitro Retinal Photoreceptor Degeneration
info:eu-repo/semantics/article