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2014-11-14T12:37:39Z urn:hdl:10324/7158 Evidence for two types of nicotinic receptors in the cat carotid body chemoreceptor cells Obeso Cáceres, Ana María de la Luz Gómez Niño, María Ángeles Almaraz Gómez, Laura Dinger, Bruce Fidone, Salvatore González Martínez, Constancio Neurofisiología Receptores celulares Producción Científica Current concepts on the location and functional significance of nicotinic receptors in the carotid body rest on a-bungarotoxin binding and autoradiographic studies. Using an in vitro preparation of the cat carotid body whose catecholamine deposits have been labeled by prior incubation with the tritiated natural precursor w3Hxtyrosine, we have found that nicotine induces release of w3Hxcatecholamines in a dose-dependent manner IC50s9.81 mM.. We also found that mecamylamine 50 mM. completely abolished the nicotine-induced release, while a-bungarotoxin 100 nM; f20 times its binding Kd. only reduced the release by 56%. These findings indicate that chemoreceptor cells, and perhaps other carotid body structures, contain nicotinic receptors that are not sensitive to a-bungarotoxin and force a revision of the current concepts on cholinergic mechanisms in the carotid body chemoreception. 2014-11-14T12:37:39Z 2014-11-14T12:37:39Z 1997 info:eu-repo/semantics/article Brain Research 754 1997. 298–302 0006-8993 http://uvadoc.uva.es/handle/10324/7158 10.1016/s0006-8993(97)00185-6 298 302 Brain Research 754 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/4.0/ Attribution-NonCommercial-NoDerivatives 4.0 International Elsevier