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oai:uvadoc.uva.es:10324/725362025-03-26T19:10:04Zcom_10324_1134com_10324_931com_10324_894col_10324_1213

00925njm 22002777a 4500 dc Girotti ., Alessandra author Reguera Gómez, Javier author Arias Vallejo, Francisco Javier author Alonso Rodrigo, Matilde author Testera Gorgojo, Ana María author Rodríguez Cabello, José Carlos author 2004 The pH-responsive elastin-like polymers, [(PGVGV)2−(PGEGV)−(PGVGV)2]n with n = 5, 9, 15, 30, 45, were obtained using genetic engineering and microbial protein expression. These were intended to study the effects of the molecular weight (MW) on the properties of their inverse temperature transition (ITT) and its dependence on pH. As a result, the transition temperature decreased and the transition enthalpy increased as the molecular weight increased, especially for the lowest MWs. More strikingly, the apparent pKa for the γ-carboxyl residue of the glutamic acid also depends on MW. The apparent pKa is lower for lower MWs. In summary, the modification in the ITT caused by changes in MW is similar to the one caused by changes in the mean polarity of the polymer as described in the literature. A reduction in the molecular weight is equivalent to a decrease in the mean hydrophobicity of the polymer. Macromolecules 37, 3396-3400 (2004) https://uvadoc.uva.es/handle/10324/72536 10.1021/ma035603k 3396 3400 37 Influence of the molecular weight on the inverse temperature transition of a model genetically engineered elastin-like pH-responsive polymer