2026-04-27T12:58:04Zhttps://uvadoc.uva.es/oai/request

oai:uvadoc.uva.es:10324/726052025-09-10T09:40:23Zcom_10324_1142com_10324_931com_10324_894col_10324_1258

Guerra, Borja Fuentes Nieto, Teresa Delgado Guerra, Safira Guadalupe Grau, Amelia Olmedillas, Hugo Santana, Alfredo Ponce González, Jesús Gustavo Dorado, Cecilia Calbet, José A. L. 2024-12-16T11:57:24Z 2024-12-16T11:57:24Z 2008 PLoS One, 2008, vol. 3, n. 10, e3466 https://uvadoc.uva.es/handle/10324/72605 10.1371/journal.pone.0003466 e3466 10 PLoS ONE 3 1932-6203 To determine if there is a gender dimorphism in the expression of leptin receptors (OB-R170, OB-R128 and OB-R98) and the protein suppressor of cytokine signaling 3 (SOCS3) in human skeletal muscle, the protein expression of OB-R, perilipin A, SOCS3 and alpha-tubulin was assessed by Western blot in muscle biopsies obtained from the m. vastus lateralis in thirty-four men (age = 27.1±6.8 yr) and thirty-three women (age = 26.7±6.7 yr). Basal serum insulin concentration and HOMA were similar in both genders. Serum leptin concentration was 3.4 times higher in women compared to men (P<0.05) and this difference remained significant after accounting for the differences in percentage of body fat or soluble leptin receptor. OB-R protein was 41% (OB-R170, P<0.05) and 163% (OB-R128, P<0.05) greater in women than men. There was no relationship between OB-R expression and the serum concentrations of leptin or 17β-estradiol. In men, muscle OB-R128 protein was inversely related to serum free testosterone. In women, OB-R98 and OB-R128 were inversely related to total serum testosterone concentration, and OB-R128 to serum free testosterone concentration. SOCS3 protein expression was similar in men and women and was not related to OB-R. In women, there was an inverse relationship between the logarithm of free testosterone and SCOS3 protein content in skeletal muscle (r = −0.46, P<0.05). In summary, there is a gender dimorphism in skeletal muscle leptin receptors expression, which can be partly explained by the influence of testosterone. SOCS3 expression in skeletal muscle is not up-regulated in women, despite very high serum leptin concentrations compared to men. The circulating form of the leptin receptor can not be used as a surrogate measure of the amount of leptin receptors expressed in skeletal muscles. eng info:eu-repo/semantics/openAccess http://creativecommons.org/publicdomain/zero/1.0/ CC0 1.0 Universal Gender dimorphism in skeletal muscle leptin receptors, serum leptin and insulin sensitivity info:eu-repo/semantics/article