2026-05-05T12:52:24Zhttps://uvadoc.uva.es/oai/request

oai:uvadoc.uva.es:10324/726082025-09-10T09:41:02Zcom_10324_1142com_10324_931com_10324_894col_10324_1258

Guerra, Borja Olmedillas, Hugo Guadalupe Grau, Amelia Ponce González, Jesús Gustavo Morales Álamo, David Fuentes Nieto, Teresa Chapinal, Esther Fernández Pérez, Leandro Pablos Velasco, Pedro de Santana, Alfredo Calbet, Jose A. L. 2024-12-16T12:30:12Z 2024-12-16T12:30:12Z 2011 Journal of Applied Physiology, 2011, vol. 111, n. 3, p. 715-725 . 8750-7587 https://uvadoc.uva.es/handle/10324/72608 10.1152/japplphysiol.00805.2010 715 3 725 Journal of Applied Physiology 111 1522-1601 Producción Científica This study was designed to determine whether sprint exercise activates signaling cascades linked to leptin actions in human skeletal muscle and how this pattern of activation may be interfered by glucose ingestion. Muscle biopsies were obtained in 15 young healthy men in response to a 30-s sprint exercise (Wingate test) randomly distributed into two groups: the fasting (n = 7, C) and the glucose group (n = 8, G), who ingested 75 g of glucose 1 h before the Wingate test. Exercise elicited different patterns of JAK2, STAT3, STAT5, ERK1/2, p38 MAPK phosphorylation, and SOCS3 protein expression during the recovery period after glucose ingestion. Thirty minutes after the control sprint, STAT3 and ERK1/2 phosphorylation levels were augmented (both, P < 0.05). SOCS3 protein expression was increased 120 min after the control sprint but PTP1B protein expression was unaffected. Thirty and 120 min after the control sprint, STAT5 phosphorylation was augmented (P < 0.05). Glucose abolished the 30 min STAT3 and ERK1/2 phosphorylation and the 120 min SOCS3 protein expression increase while retarding the STAT5 phosphorylation response to sprint. Activation of these signaling cascades occurred despite a reduction of circulating leptin concentration after the sprint. Basal JAK2 and p38 MAPK phosphorylation levels were reduced and increased (both P < 0.05), respectively, by glucose ingestion prior to exercise. During recovery, JAK2 phosphorylation was unchanged and p38 MAPK phosphorylation was transiently reduced when the exercise was preceded by glucose ingestion. In conclusion, sprint exercise performed under fasting conditions is a leptin signaling mimetic in human skeletal muscle. application/pdf eng info:eu-repo/semantics/openAccess http://creativecommons.org/publicdomain/zero/1.0/ CC0 1.0 Universal Is sprint exercise a leptin signaling mimetic in human skeletal muscle? info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://journals.physiology.org/doi/full/10.1152/japplphysiol.00805.2010 SI