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oai:uvadoc.uva.es:10324/726452025-09-10T09:38:53Zcom_10324_1142com_10324_931com_10324_894col_10324_1258

Guerra, Borja Ponce González, Jesús Gustavo Morales Álamo, David Guadalupe Grau, Amelia Kiilerich, Kristian Fuentes Nieto, Teresa Ringholm, Stine Biensø, Rasmus Sjørup Santana, Alfredo Lundby, Carsten Pilegaard, Henriette Calbet, José A. L. 2024-12-17T09:14:42Z 2024-12-17T09:14:42Z 2014 Guerra B, Ponce-González JG, Morales-Alamo D, Guadalupe-Grau A, Kiilerich K, Fuentes T, Ringholm S, Biensø RS, Santana A, Lundby C, Pilegaard H, Calbet JA. Leptin signaling in skeletal muscle after bed rest in healthy humans. Eur J Appl Physiol. 2014 Feb;114(2):345-57. doi: 10.1007/s00421-013-2779-4. Epub 2013 Nov 29. PMID: 24292882. 1439-6319 https://uvadoc.uva.es/handle/10324/72645 10.1007/s00421-013-2779-4 345 2 357 European Journal of Applied Physiology 114 1439-6327 Producción Científica This study aimed at determining the effects of bed rest on the skeletal muscle leptin signaling system. Deltoid and vastus lateralis muscle biopsies and blood samples were obtained from 12 healthy young men (mean ± SD, BMI 22.8 ± 2.7 kg/m(2)) before and after 7 days of bed rest. Leptin receptor isoforms (OB-Rs), suppressor of cytokine signaling 3 (SOCS3) and protein tyrosine phosphatase 1B (PTP1B) protein expression and signal transducer and activator of transcription 3 (STAT3) phosphorylation were analyzed by Western blot. After bed rest basal insulin concentration was increased by 53 % (P < 0.05), the homeostasis model assessment (HOMA) by 40 % (P < 0.05), and serum leptin concentration by 35 % (P < 0.05) with no changes in body fat mass. Although the soluble isoform of the leptin receptor (s-OBR) remained unchanged, the molar excess of leptin over sOB-R was increased by 1.4-fold after bed rest (P < 0.05). OB-Rs and SOCS3 protein expression, and STAT3 phosphorylation level remained unaffected in deltoid and vastus lateralis by bed rest, as PTP1B in the deltoid. PTP1B was increased by 90 % with bed rest in the vastus lateralis (P < 0.05). There was a linear relationship between the increase in vastus lateralis PTP1B and the increase in both basal insulin concentrations (r = 0.66, P < 0.05) and HOMA (r = 0.68, P < 0.05) with bed rest. One week of bed rest is associated with increased leptin levels without augmenting STAT3 phosphorylation indicating some degree of leptin resistance in skeletal muscle, which can be explained, at least in part, by an elevation of PTP1B protein content in the vastus lateralis muscle. Ministerio de Ciencia e Innovación (BFU2006-13784 and FEDER) y FUncIS (PI/10/07). application/pdf eng Springer info:eu-repo/semantics/openAccess ringer-Verlag Berlin Heidelberg. T European Journal of Applied Physiology 114, 345–357 (2014) European Journal of Applied Physiology 114, 345–357 (2014) Leptin signaling in skeletal muscle after bed rest in healthy humans info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion SI