2026-04-27T19:58:57Zhttps://uvadoc.uva.es/oai/request

oai:uvadoc.uva.es:10324/732582025-09-09T06:08:43Zcom_10324_1173com_10324_931com_10324_894col_10324_1371

00925njm 22002777a 4500 dc Morales Palomo, Sergio author Tomás Pejó, Elia author González Fernández, María Cristina author 2024 Some oleaginous yeasts have the ability to produce microbial oils from alternative carbon sources, such as short-chain fatty acids (SCFAs). Nevertheless, there is still a lack of information about the possible effects that media nutrients have on yeast metabolisms when using SCFAs. For instance, inorganic phosphate (PO43-) has been reported to promote yeast growth in literature but its chelating effect over other elements such as calcium (Ca2+) is often not considered in fermentation processes while limitation of nitrogen is probably the most studied. Attending at the need to better understand the role of PO43-, this work assessed the lipid production capacity of Yarrowia lipolytica ACA DC 50109, both in synthetic and real SCFAs-rich media, at different SCFAs concentrations and PO43-:Ca2+ ratios. Reducing PO43-:Ca2+ ratio was identified to be an important factor to improve yeast growth, reaching the highest lipid content (52.7 ± 0.9 % w/w) and lipid yield (0.31 ± 0.01 w/w) in media without PO43-. These results demonstrated the importance of Ca2+ availability in the medium and nutrients interactions in yeast growth that are often underestimated. Environmental Technology & Innovation, noviembre 2024, vol. 36, 103767 2352-1864 https://uvadoc.uva.es/handle/10324/73258 10.1016/j.eti.2024.103767 103767 Environmental Technology & Innovation 36 Phosphate chelation over calcium impacts yeast growth and lipid production from short-chain fatty acids-rich media