2026-04-26T21:56:33Zhttps://uvadoc.uva.es/oai/requestoai:uvadoc.uva.es:10324/738642025-01-17T07:12:24Zcom_10324_1134com_10324_931com_10324_894col_10324_1213
UVaDOC
author
Girotti ., Alessandra
author
González Valdivieso, Juan
author
Santos García, María Mercedes
author
Martín Maroto, Laura
author
Arias Vallejo, Francisco Javier
2025-01-15T10:51:55Z
2025-01-15T10:51:55Z
2020
International Journal of Biological Macromolecules, diciembre 2020, vol. 164, p. 1640-1648
0141-8130
https://uvadoc.uva.es/handle/10324/73864
10.1016/j.ijbiomac.2020.08.004
1640
1648
International Journal of Biological Macromolecules
164
One of the main goals in both tissue engineering and regenerative medicine is to design innovative synthetic scaffolds that can simulate and control the communication pathways between cells and the extracellular matrix (ECM). In this context, we describe herein the characterization of protein polymer, a recombinant elastin-like recombinamer (ELR) designed for developing tissue-engineered devices for use in vascular regeneration. This ELR is composed of an elastin-like backbone that contains a fibronectin domain, which provides specific, endothelial cell adhesion, and a protease target domain directed towards specific proteases involved in ECM remodeling. We also compare the specific response of endothelial and fibroblast cells to ELR scaffolds and show that cell adhesion and spreading on this ELR is significantly higher for endothelial cells than for fibroblasts. The reactivity of this polymer and its hydrogels to specific enzymatic degradation is demonstrated in vitro. As with natural elastin, enzymatic hydrolysis of the ELR produces elastin-derived peptides, or “matrikines”, which, in turn, are potentially able to regulate important cell activities.
eng
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Functional characterization of an enzymatically degradable multi-bioactive elastin-like recombinamer
info:eu-repo/semantics/article
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URL
https://uvadoc.uva.es/bitstream/10324/73864/1/functional-characterization-elastin-like-recombinamer_preprint.pdf
File
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09f1e8f06f9676d4849baa4a2b125ee1
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application/pdf
functional-characterization-elastin-like-recombinamer_preprint.pdf