2026-05-05T09:23:51Zhttps://uvadoc.uva.es/oai/request
oai:uvadoc.uva.es:10324/739112025-02-06T09:21:39Zcom_10324_1134com_10324_931com_10324_894col_10324_1213
UVaDOC author Álvarez Illera, María Pilar author García Casas, Paloma author Arias del Val, Jessica author Fonteriz García, Rosalba Inés author Álvarez Martín, Javier author Montero, Mayte 2025-01-16T09:00:09Z 2025-01-16T09:00:09Z 2017 Alvarez-Illera P, García-Casas P, Arias-Del-Val J, Fonteriz RI, Alvarez J, Montero M. Pharynx mitochondrial [Ca2+] dynamics in live C. elegans worms during aging. Oncotarget. 2017 Jun 22;8(34):55889-55900. doi: 10.18632/oncotarget.18600. PMID: 28915560; PMCID: PMC5593531. https://uvadoc.uva.es/handle/10324/73911 10.18632/oncotarget.18600 55889 34 55900 Oncotarget 8 1949-2553 Progressive decline in mitochondrial function is generally considered one of the hallmarks of aging. We have expressed a Ca2+ sensor in the mitochondrial matrix of C. elegans pharynx cells and we have measured for the first time mitochondrial [Ca2+] ([Ca2+]M) dynamics in the pharynx of live C. elegans worms during aging. Our results show that worms stimulated with serotonin display a pharynx [Ca2+]M oscillatory kinetics that includes both high frequency oscillations (up to about 1Hz) and very prolonged "square-wave" [Ca2+]M increases, indicative of energy depletion of the pharynx cells. Mitochondrial [Ca2+] is therefore able to follow "beat-to-beat" the fast oscillations of cytosolic [Ca2+]. The fast [Ca2+]M oscillations kept steady frequency values during the whole worm life, from 2 to 12 days old, but the height and width of the peaks was progressively reduced. [Ca2+]M oscillations were also present with similar kinetics in respiratory chain complex I nuo-6 mutant worms, although with smaller height and frequency than in the controls, and larger width. In summary, Ca2+ fluxes in and out of the mitochondria are relatively well preserved during the C. elegans life, but there is a clear progressive decrease in their magnitude during aging. Moreover, mitochondrial Ca2+ fluxes were smaller in nuo-6 mutants with respect to the controls at every age and decreased similarly during aging. eng CC0 1.0 Universal Pharynx mitochondrial [Ca2+] dynamics in live C. elegans worms during aging info:eu-repo/semantics/article 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URL https://uvadoc.uva.es/bitstream/10324/73911/1/2017%20Pharynx%20mitochondrial%20%5bCa%202%2b%5d%20dynamics%20in%20live%20C.%20elegans%20worms%20during%20aging.pdf File MD5 fd83625f05047252dbaa863f2ff352e8 1718937 application/pdf 2017 Pharynx mitochondrial [Ca 2+] dynamics in live C. elegans worms during aging.pdf