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UVaDOC author Sánchez, Manuel author Secades, Lorena author Bordallo, Carmen author Meana González, Clara author Rubín, José Manuel author Cantabrana, Begoña author Bordallo, Javier 2025-01-22T13:02:56Z 2025-01-22T13:02:56Z 2009 Journal of Cardiovascular Pharmacology, octubre 2009, vol. 54, n. 4, p. 310-318 0160-2446 https://uvadoc.uva.es/handle/10324/74261 10.1097/FJC.0b013e3181b6e57f 310 4 318 Journal of Cardiovascular Pharmacology 54 Androgens produce acute vasodilation of systemic, pulmonary, and coronary arteries in several mammal preparations and increase cardiomyocyte contractility. A decrease of the spontaneous beating of sinoatrial cells has also been described. The aim of this study was to characterize the direct effect of 5a-dihydrotestosterone on the spontaneous chronotropism and inotropism in the same preparation as an approach to establish the effect on cardiac output and their mechanism of action. The effects were studied on isolated right atria of Wistar rats placed in an organ bath in Tyrode solution at 37 C and bubbled with carbogen. In male rats, the acute administration of 5a-dihydrotestosterone, a nonaromatizable derivate of testosterone, elicited a positive inotropism, which was associate with a negative chronotropism. As reported in the left atria, polyamines and b-adrenoceptors played a role in 5a-dihydrotestosterone–elicited positive inotropism because the effect was antagonized by a-difluoromethylornithine, an inhibitor of polyamine synthesis, and atenolol, a b1-adrenoceptor blocker, but not on the negative effect on chronotropism. The androgen increased the sinoatrial node recovery time, suggesting an effect on the mechanisms of spontaneous diastolic depolarization involved in atria pacemaking. These effects of 5a-dihydrotestosterone are not hormonally regulated because they are similarly produced in estrogenized females and gonadectomised male and female rats. These results suggest that the androgen could acutely improve cardiac performance. eng Role of Polyamines and cAMP-dependent Mechanisms on 5α-dihydrotestosterone-elicited Functional Effects in Isolated Right Atria of Rat info:eu-repo/semantics/article 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URL https://uvadoc.uva.es/bitstream/10324/74261/1/03.%202009.%20JCVP.%20Role%20of%20polyamines%20and%20cAMP-dependent%20mechanisms%20on%205a-DHT-elicited%20functional%20effects%20in%20isolated%20right%20atria%20of%20rat.pdf File MD5 5f5b6ed0d138506cd72733bdc4917ff7 306333 application/pdf 03. 2009. JCVP. Role of polyamines and cAMP-dependent mechanisms on 5a-DHT-elicited functional effects in isolated right atria of rat.pdf