2026-04-23T20:26:59Zhttps://uvadoc.uva.es/oai/request

oai:uvadoc.uva.es:10324/747082025-05-05T12:06:02Zcom_10324_1132com_10324_931com_10324_894col_10324_1205

Kaiser, Karol Gyllborg, Daniel Procházka, Jan Salašová, Alena Kompaníková, Petra Molina, Francisco Lamus Laguna-Goya, Rocio Radaszkiewicz, Tomasz Harnoš, Jakub Procházková, Michaela Potěšil, David Barker, Roger A. Gato Casado, Ángel Luis Zdráhal, Zbyněk Sedláček, Radislav Arenas, Ernest Villaescusa, J. Carlos Bryja, Vítězslav 2025-01-31T09:53:07Z 2025-01-31T09:53:07Z 2019 WNT5A is transported via lipoprotein particles in the cerebrospinal fluid to regulate hindbrain morphogenesis. Nat Commun. 2019 Apr 2;10(1):1498 2041-1723 https://uvadoc.uva.es/handle/10324/74708 10.1038/s41467-019-09298-4 1498 1 1498 Nature Communications 10 2041-1723 Producción Científica WNTs are lipid-modified proteins that control multiple functions in development and disease via short- and long-range signaling. However, it is unclear how these hydrophobic molecules spread over long distances in the mammalian brain. Here we show that WNT5A is produced by the choroid plexus (ChP) of the developing hindbrain, but not the telencephalon, in both mouse and human. Since the ChP produces and secretes the cerebrospinal fluid (CSF), we examine the presence of WNT5A in the CSF and find that it is associated with lipoprotein particles rather than exosomes. Moreover, since the CSF flows along the apical surface of hindbrain progenitors not expressing Wnt5a, we examined whether deletion of Wnt5a in the ChP controls their function and find that cerebellar morphogenesis is impaired. Our study thus identifies the CSF as a route and lipoprotein particles as a vehicle for long-range transport of biologically active WNT in the central nervous system. application/pdf spa Nature Publishing Group info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/ The Author(s) 2019 Atribución 4.0 Internacional Embriología Experimental WNT5A signaling Cerebrospinal fluid (CSF) Lipoprotein transport Hindbrain morphogenesis Neurodevelopment 2401.07 Embriología Animal WNT5A is transported via lipoprotein particles in the cerebrospinal fluid to regulate hindbrain morphogenesis info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.nature.com/articles/s41467-019-09298-4 SI