2026-04-14T16:00:11Zhttps://uvadoc.uva.es/oai/request

oai:uvadoc.uva.es:10324/761742025-11-13T09:51:47Zcom_10324_1183com_10324_931com_10324_894col_10324_1396

Mosquera, Sandra Ginésy, Mireille Amin, Huma Bocos Asenjo, Irene Teresa Niño Sánchez, Jonatan Diez Hermano, Sergio Díez Casero, Julio Javier 2025-07-01T08:47:21Z 2025-07-01T08:47:21Z 2025 Journal of Integrative Plant Biology, 2025, vol. 67, n. 3. p. 801-825 1672-9072 https://uvadoc.uva.es/handle/10324/76174 10.1111/JIPB.13848 801 3 825 Journal of Integrative Plant Biology 67 1744-7909 RNA interference (RNAi)-based control technologies are gaining popularity as potential alternatives to synthetic fungicides in the ongoing effort to manage plant pathogenic fungi. Among these methods, spray-induced gene silencing (SIGS) emerges as particularly promising due to its convenience and feasibility for development. This approach is a new technology for plant disease management, in which double-stranded RNAs (dsRNAs) targeting essential or virulence genes are applied to plants or plant products and subsequently absorbed by plant pathogens, triggering a gene silencing effect and the inhibition of the infection process. Spray-induced gene silencing has demonstrated efficacy in laboratory settings against various fungal pathogens. However, as research progressed from the laboratory to the greenhouse and field environments, novel challenges arose, such as ensuring the stability of dsRNAs and their effective delivery to fungal targets. Here, we provide a practical guide to SIGS for the control of plant pathogenic fungi. This guide outlines the essential steps and considerations needed for designing and assessing dsRNA molecules. It also addresses key challenges inherent to SIGS, including delivery and stability of dsRNA molecules, and how nanoencapsulation of dsRNAs can aid in overcoming these obstacles. Additionally, the guide underscores existing knowledge gaps that warrant further research and aims to provide assistance to researchers, especially those new to the field, encouraging the advancement of SIGS for the control of a broad range of fungal pathogens. eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/ © 2025 The Author(s) Atribución 4.0 Internacional Fungal plant pathogens Silenciamiento genético inducido por pulverización Spray‐ induced gene silencing (SIGS) Spray‐induced gene silencing to control plant pathogenic fungi: A step‐by‐step guide info:eu-repo/semantics/article