2026-05-05T18:37:18Zhttps://uvadoc.uva.es/oai/request

oai:uvadoc.uva.es:10324/769212025-10-08T10:01:34Zcom_10324_1181com_10324_931com_10324_894col_10324_1387

Complex immune network and regional consistency in the human gastric mucosa revealed by high-resolution spectral cytometry De Prado, Ángel Cal Sabater, Paloma De La Fiz López, Aida Izquierdo, Sandra Corrales, Daniel Pérez-Cózar, Francisco H-Vázquez, Juan Arribas Rodríguez, Elisa Perez-Segurado, Cándido Muñoz, Álvaro Martín Garrote Adrados, José Antonio Arranz Sanz, Eduardo Marañón, Concepción Cuesta Sancho, Sara Fernández Salazar, Luis Ignacio Bernardo Ordiz, David Biomedicina Inmunología The immune cellular landscape from the gastric mucosa remains largely unknown despite its relevance in several inflammatory conditions. Human gastric biopsies were obtained from the antrum, body and incisura from 10 individuals to obtain lamina propria mononuclear cells that were further characterized by spectral cytometry. Phenotypic hierarchical analyses identified a total of 52 different immune cell subsets within the human gastric mucosa revealing that T-cells (> 60%) and NK cells (> 20%) were the main populations. Within T-cells, CD4+ and CD8+ were equally represented with both subsets displaying mainly a memory and effector phenotype. NK cells, on the contrary, were largely of the early phenotype. No regional differences were observed for any subsets among the 3 locations. Following unsupervised analysis, a total of 82 clusters were found. Again, no differences were observed amongst locations although a great degree of inter-individual variability was found, largely influenced by the presence of H. pylori infection and dyspepsia. We have unraveled the human gastric immune cellular subset composition and a unique interindividual immune fingerprint with no inter-regional variations. 2025-07-29T07:47:18Z 2025-07-29T07:47:18Z 2025-07-29T07:47:18Z 2024 info:eu-repo/semantics/article Scientific Reports, 2024, n.14, 28685 2045-2322 https://uvadoc.uva.es/handle/10324/76921 10.1038/s41598-024-78908-z 1 Scientific Reports 14 2045-2322 eng https://www.nature.com/articles/s41598-024-78908-z# info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/4.0/ Attribution-NonCommercial-NoDerivatives 4.0 Internacional Scientific Reports