2026-04-28T19:36:21Zhttps://uvadoc.uva.es/oai/request

oai:uvadoc.uva.es:10324/770002025-09-04T11:34:31Zcom_10324_1183com_10324_931com_10324_894col_10324_1396

Using Bursaphelenchus mucronatus to demonstrate the potential nematicidal effect of Beauveria bassiana on pine wood nematode (Bursaphelenchus xylophilus) under in vivo conditions Sánchez Gómez, Tamara Zamora, Paula Díez Casero, Julio Javier Herrero Villacorta, Baudilio Poveda Arias, Jorge Martín García, Jorge Producción Científica Context Pine wilt disease (PWD) is a serious threat for conifer forests worldwide. It is caused by Bursaphelenchus xylophilus, the pine wood nematode (PWN). In affected areas, eradication and subsequent disease containment measures are being implemented. The latter are, to date, based on control strategies for the insect vectors (Monochamus spp.) and on screening for genetic resistance in tree hosts. However, an integrated pest management strategy which also implements nematode control is still not fully developed. Aims This study aimed to use Bursaphelenchus mucronatus, as an organism on which to demonstrate the nematicidal potential of Beauveria bassiana, an entomopathogenic fungus successfully tested on Monochamus spp., on PWN under in vivo conditions. Methods To this end, a pathosystem was built to simulate these conditions and to bring the nematode B. mucronatus, the insect vector, and the fungus into contact. Results The results show (i) very similar responses of the two nematodes confronted to the fungus and its mycotoxin beauvericin under in vitro conditions and (ii) a remarkable antagonistic effect of B. bassiana on B. mucronatus also on the abovementioned pathosystem (in vivo conditions). Conclusion Our findings have significant implications for the pine wilt disease control. In particular, this study demonstrates the potential of B. bassiana as a biological control tool to be implemented in a future integrated disease management strategy. 2025-07-30T10:13:35Z 2025-07-30T10:13:35Z 2025 info:eu-repo/semantics/article Annals of Forest Science, 2025, vol. 82, art. 4 https://uvadoc.uva.es/handle/10324/77000 10.1186/s13595-025-01274-x 1 Annals of Forest Science 82 1297-966X eng https://annforsci.biomedcentral.com/articles/10.1186/s13595-025-01274-x info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/ © The Authors Atribución 4.0 Internacional BMC SI