2026-04-22T04:28:15Zhttps://uvadoc.uva.es/oai/request

oai:uvadoc.uva.es:10324/842272026-04-21T19:21:53Zcom_10324_35294com_10324_952com_10324_894col_10324_35295

Torres Franco, Andrés Felipe Rodríguez Rodríguez, Elisa Carita Gonçalves, José Manuel González, María José Muñoz Torre, Raúl García Encina, Pedro Antonio Díaz Villalobos, Israel 2026 Producción Científica Wastewater Treatment Plants (WWTPs) based on activated sludge (AS) processes have demonstrated moderate capacity to remove viruses from wastewater. However, few studies have systematically assessed viruses along water and sludge treatment lines and their associations with Antibiotic-Resistant Genes (ARGs). In this work, the fate, distribution and possible interactions of RNA from SARS-CoV-2 and coliphage MS2 with ARGs in a full-scale AS WWTP were investigated. Total reductions of SARS-CoV-2 and MS2 RNA (2.5 and 2.4 log10 units, respectively) were achieved mainly in the water treatment line. In contrast, lower total reductions (0.7–1.5 log10 units) of 16S rRNA, class 1 integron-integrase (intI1), sulfonamide-resistant dihydropteroate synthase (sul1) and tetracycline resistance protein (tetO) were mainly achieved during sludge treatment. In addition to the water treatment line, sludge stabilisation was a critical barrier to controlling viral RNA and ARGs in the outflows. Amplification of intI1 and sul1 seemed to occur in the AS reactors, potentially associated with bacteriophage presence in these units. Thus, the participation of bacteriophages in the amplification of ARGs in AS systems should not be ruled out. RNA viruses and ARGs should be more frequently determined in WWTPs to assess and prevent phage-mediated amplification of ARGs. application/pdf https://uvadoc.uva.es/handle/10324/84227 eng Elsevier 3308 Ingeniería y Tecnología del Medio Ambiente Viral RNA and antibiotic resistance genes associations in a full-scale activated sludge wastewater treatment plant info:eu-repo/semantics/article TEXT UVaDOC. Repositorio Documental de la Universidad de Valladolid Hispana