2026-04-14T18:32:00Zhttps://uvadoc.uva.es/oai/request

oai:uvadoc.uva.es:10324/90422025-02-19T08:14:55Zcom_10324_1133com_10324_931com_10324_894col_10324_1209

Validation of an experimental animal model for corneal additive surgery Ibares Frías, Lucía Gallego Muñoz, Patricia Cantalapiedra Rodríguez, Roberto Valsero Blanco, María Cruz Mar Sardaña, Santiago Merayo Lloves, Jesús Martínez García, María del Carmen Cirugía oftalmológica Producción Científica Purpose: To assess the hen cornea as a model for training and future wound healing studies after implantation of intrastromal corneal ring segments (ICRS) by clinical and optical outcomes. Setting: University of Valladolid, Valladolid, Spain. Design: Experimental study. Methods: One 90°, 150-μm thick polymethyl methacrylate Ferrara ICRS segment was manually implanted at 70-80% depth of 192 Gallus domesticus corneas. Clinical follow-up for 6 months included monitoring corneal thickness, epithelial wound closure, edema, haze, and the location and severity of deposits. The refractive state was also measured. After each animal was euthanized, corneas were processed for direct transmittance and histological analysis. Results: Complications were present in 16% of the eyes. Epithelial wound closure was completed at 3 ± 2 days. A slight corneal edema in the channel site was present for the first 15 days. All corneas had deposits by 4 months located along the inner, outer curvatures and under the segments. Corneal haze was present only at the incision site. ICRS induced hyperopic changes in the refractive state without changes in direct transmitance of central cornea. New cells and extracellular matrix were present around the segment where deposits were seen on clinical follow-up. Conclusions: With hen as an animal model, ICRS were implanted in a precise and reproducible way after a learning curve. Similar to humans, the follow-up period during the first 6 months after implantation showed fast wound closure, deposits, and haze at the incision site. ICRS in hens also reduced the refractive power withoutaffecting the central cornea. 2015-03-06T09:16:39Z 2015-03-06T09:16:39Z 2014 info:eu-repo/semantics/article J Clin Exp Ophthalmol, 2014, vol. 5, no 360 2155-9570 http://uvadoc.uva.es/handle/10324/9042 10.4172/2155-9570.1000360 360 J Clin Experimental Ophthalmology 5 eng Attribution-NonCommercial-NoDerivatives 4.0 International info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/4.0/ application/pdf OMICS Publishing Group https://uvadoc.uva.es/bitstream/10324/9042/6/Carmen%20Mart%c3%adnez%202%20PD-209.pdf.jpg Hispana TEXT http://creativecommons.org/licenses/by-nc-nd/4.0/ UVaDOC. Repositorio Documental de la Universidad de Valladolid http://uvadoc.uva.es/handle/10324/9042