2024-03-28T11:51:49Zhttps://uvadoc.uva.es/oai/requestoai:uvadoc.uva.es:10324/244242021-06-24T07:27:06Zcom_10324_22821com_10324_954com_10324_894col_10324_22822
2017-07-14T11:12:16Z
urn:hdl:10324/24424
Human adipose derived stem cells are superior to human osteoblasts (HOB) in bone tissue engineering on a collagen-fibroin-ELR blend
Sayin, Esen
Hama Rashid, Rosti
Rodríguez Cabello, José Carlos
Elsheikh Erkan, Ahmed
Türker Baran, Erkan
Hasirci, Vasif
Producción Científica
The ultrastructure of the bone provides a unique mechanical strength against compressive, torsional and tensional stresses. An elastin-like recombinamer (ELR) with a nucleation sequence for hydroxyapatite was incorporated into films prepared from a collagen – silk fibroin blend carrying microchannel patterns to stimulate anisotropic osteogenesis. SEM and fluorescence microscopy showed the alignment of adipose-derived stem cells (ADSCs) and the human osteoblasts (HOBs) on the ridges and in the grooves of microchannel patterned collagen-fibroin-ELR blend films. The Young's modulus and the ultimate tensile strength (UTS) of untreated films were 0.58 ± 0.13 MPa and 0.18 ± 0.05 MPa, respectively. After 28 days of cell culture, ADSC seeded film had a Young's modulus of 1.21 ± 0.42 MPa and UTS of 0.32 ± 0.15 MPa which were about 3 fold higher than HOB seeded films. The difference in Young's modulus was statistically significant (p: 0.02). ADSCs attached, proliferated and mineralized better than the HOBs. In the light of these results, ADSCs served as a better cell source than HOBs for bone tissue engineering of collagen-fibroin-ELR based constructs used in this study. We have thus shown the enhancement in the tensile mechanical properties of the bone tissue engineered scaffolds by using ADSCs.
2017-07-14T11:12:16Z
2017-07-14T11:12:16Z
2017
info:eu-repo/semantics/article
Bioactive Materials, 2017, Volume 2, Issue 2, Pages 71-81
http://uvadoc.uva.es/handle/10324/24424
https://doi.org/10.1016/j.bioactmat.2017.04.001
eng
http://www.sciencedirect.com/science/article/pii/S2452199X17300038
info:eu-repo/grantAgreement/EC/H2020/642687
info:eu-repo/grantAgreement/EC/H2020/646075
info:eu-repo/grantAgreement/EC/FP7/278557
info:eu-repo/grantAgreement/EC/FP7/317306
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
Attribution-NonCommercial-NoDerivatives 4.0 International
Royal Society of Chemistry