2024-03-28T14:39:33Zhttps://uvadoc.uva.es/oai/requestoai:uvadoc.uva.es:10324/408302021-06-23T11:47:34Zcom_10324_1183com_10324_931com_10324_894col_10324_1396
2020-05-07T13:04:51Z
urn:hdl:10324/40830
Fungal Communities Associated with Bark Beetles in Pinus radiata Plantations in Northern Spain Affected by Pine Pitch Canker, with Special Focus on Fusarium Species
Bezos García, Diana
Martínez Álvarez, Pablo
Sanz Ros, Antonio Vicente
Martín García, Jorge
Fernández Fernández, María Mercedes
Díez Casero, Julio Javier
Producción Científica
Fusarium spp., as well as other endophytic or pathogenic fungi that form communities, have
been reported to be phoretically associated with bark beetles (Coleoptera; Scolytinae) worldwide.
This applies to Fusarium circinatum Nirenberg and O’Donnell, the causal agent of pine pitch canker
(PPC), which threatens Pinus radiata D. Don plantations in northern Spain. The main objective of
this study was to study the fungal communities associated with bark beetles and their galleries in
stands affected by PPC, with special attention given to Fusarium species. Funnel traps and logs were
placed in a P. radiata plot known to be affected by F. circinatum. The traps were baited with different
attractants: four with (E)-pityol and six with ethanol and α-Pinene. In addition, fresh green shoots
with Tomicus piniperda L. feeding galleries were collected from the ground in 25 P. radiata plots affected
by PPC. Extracts of whole insects and gallery tissues were plated on agar medium to isolate and
identify the associated fungi. A total of 24 different fungal species were isolated from the bark beetle
galleries constructed in logs and shoots, while 18 were isolated from the insect exoskeletons. Ten
different Fusarium species were isolated from tissue and insects. Fusarium circinatum was isolated
from bark beetle exoskeletons (1.05% of the Pityophthorus pubescens Marsham specimens harboured
F. circinatum) and from the galleries (3.5% of the T. piniperda feeding galleries harboured the pathogen).
The findings provide information about the fungal communities associated with bark beetles in
P. radiata stands in northern Spain.
2020-05-07T13:04:51Z
2020-05-07T13:04:51Z
2018
info:eu-repo/semantics/article
Forests, 2018, vol. 9, n. 11. 20 p.
1999-4907
http://uvadoc.uva.es/handle/10324/40830
10.3390/f9110698
eng
https://www.mdpi.com/1999-4907/9/11/698
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
© 2018 MDPI
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