2024-03-28T09:20:41Zhttps://uvadoc.uva.es/oai/requestoai:uvadoc.uva.es:10324/408392021-06-23T11:47:48Zcom_10324_1183com_10324_931com_10324_894col_10324_1396
Ioos, Renaud
Aloi, Francesco
Piškur, Barbara
Guinet, Cécile
Mullett, Martin
Berbegal, Mónica
Bragança, Helena
Cacciola, Santa Olga
Oskay, Funda
Cornejo, Carolina
Adamson, Kalev
Douanla-Meli, Clovis
Kačergius, Audrius
Martínez Álvarez, Pablo
Nowakowska, Justyna Anna
Luchi, Nicola
Vettraino, Anna Maria
Ahumada, Rodrigo
Pasquali, Matias
Fourie, Gerda
Kanetis, Loukas
Alves, Artur
Ghelardini, Luisa
Dvořák, Miloň
Sanz Ros, Antonio Vicente
Díez Casero, Julio Javier
Baskarathevan, Jeyaseelan
Aguayo, Jaime
2020-05-11T12:05:06Z
2020-05-11T12:05:06Z
2019
Scientific Reports, 2019, vol. 9. 17 p.
2045-2322
http://uvadoc.uva.es/handle/10324/40839
10.1038/s41598-019-44672-8
Producción Científica
Fusarium circinatum is a harmful pathogenic fungus mostly attacking Pinus species and also
Pseudotsuga menziesii, causing cankers in trees of all ages, damping-of in seedlings, and mortality in
cuttings and mother plants for clonal production. This fungus is listed as a quarantine pest in several
parts of the world and the trade of potentially contaminated pine material such as cuttings, seedlings or
seeds is restricted in order to prevent its spread to disease-free areas. Inspection of plant material often
relies on DNA testing and several conventional or real-time PCR based tests targeting F. circinatum are
available in the literature. In this work, an international collaborative study joined 23 partners to assess
the transferability and the performance of nine molecular protocols, using a wide panel of DNA from 71
representative strains of F. circinatum and related Fusarium species. Diagnostic sensitivity, specifcity
and accuracy of the nine protocols all reached values >80%, and the diagnostic specifcity was the only
parameter difering signifcantly between protocols.The rates of false positives and of false negatives
were computed and only the false positive rates difered signifcantly, ranging from 3.0% to 17.3%.The
diference between protocols for some ofthe performance values were mainly due to cross-reactions
with DNA from non-target species, which were either not tested or documented in the original articles.
Considering that participating laboratories were free to use their own reagents and equipment, this
study demonstrated that the diagnostic protocols for F. circinatum were not easily transferable to endusers. More generally, our results suggest that the use of protocols using conventional or real-time PCR
outside their initial development and validation conditions should require careful characterization of
the performance data priorto use under modifed conditions (i.e. reagents and equipment). Suggestions
to improve the transfer are proposed.
European Cooperation in Science and Technology (COST Action FP1406 PINESTRENGTH)
Estonian Science Foundation (grants PSG136 and IUT21-04)
Centro de Estudios do Ambiente e do Mar (CESAM) (projects UID/AMB/50017/2013 and POCI-01- 0145- FEDER-007638)
Slovenian Research Agency (project P4-0107)
application/pdf
eng
Springer Nature
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
© 2019 Springer Nature
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Pine pitch canker
Chancro resinoso del pino
PCR-based tests
Tests PCR
Diagnóstico de enfermedad fungica
Fungal infections - Diagnosis
Enfermedades fúngicas - Diagnóstico
3106 Ciencia Forestal
Transferability of PCR-based diagnostic protocols: An international collaborative case study assessing protocols targeting the quarantine pine pathogen Fusarium circinatum
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
https://www.nature.com/articles/s41598-019-44672-8
SI