2024-03-29T01:30:39Zhttps://uvadoc.uva.es/oai/requestoai:uvadoc.uva.es:10324/450252021-06-24T07:16:24Zcom_10324_44585com_10324_954com_10324_894col_10324_44586
Chamero, Pablo
Weiss, Jan
Alonso Alonso, María Teresa
Rodríguez Prados, Macarena
Hisatsune, Chihiro
Mikoshiba, Katsuhiko
Leinders-Zufall, Trese
Zufall, Frank
2021-01-15T13:06:41Z
2021-01-15T13:06:41Z
2017
Scientific Reports, 2017, vol. 7. 15 p.
2045-2322
http://uvadoc.uva.es/handle/10324/45025
10.1038/s41598-017-09638-8
Producción Científica
Signal transduction in sensory neurons of the mammalian vomeronasal organ (VNO) involves the opening of the canonical transient receptor potential channel Trpc2, a Ca2+-permeable cation channel that is activated by diacylglycerol and inhibited by Ca2+-calmodulin. There has been a long-standing debate about the extent to which the second messenger inositol 1,4,5-trisphosphate (InsP3) and type 3 InsP3 receptor (InsP3R3) are involved in the opening of Trpc2 channels and in sensory activation of the VNO. To address this question, we investigated VNO function of mice carrying a knockout mutation in the Itpr3 locus causing a loss of InsP3R3. We established a new method to monitor Ca2+ in the endoplasmic reticulum of vomeronasal sensory neurons (VSNs) by employing the GFP-aequorin protein sensor erGAP2. We also performed simultaneous InsP3 photorelease and Ca2+ monitoring experiments, and analysed Ca2+ dynamics, sensory currents, and action potential or field potential responses in InsP3R3-deficient VSNs. Disruption of Itpr3 abolished or minimized the Ca2+ transients evoked by photoactivated InsP3, but there was virtually no effect on sensory activation of VSNs. Therefore, InsP3R3 is dispensable for primary chemoelectrical transduction in mouse VNO. We conclude that InsP3R3 is not required for gating of Trpc2 in VSNs.
Deutsche Forschungsgemeinschaf (grant CH920/2-1)
Sonderforschungsbereich 894 (project A17)
Transregio - SFB (projects P09 and P10)
Ministerio de Economía, Industria y Competitividad (grant BFU2014-53469P)
application/pdf
eng
Springer Nature
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
© 2017 Springer Nature
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Vomeronasal organ
Órgano vomeronasal
Sensory activation
Activación sensorial
Inositol trisphosphate receptor
Receptor de inositol trifosfato
Type 3 inositol 1,4,5-trisphosphate receptor is dispensable for sensory activation of the mammalian vomeronasal organ
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
https://www.nature.com/articles/s41598-017-09638-8
SI