2024-03-28T17:02:54Zhttps://uvadoc.uva.es/oai/requestoai:uvadoc.uva.es:10324/59772021-06-23T09:48:07Zcom_10324_1133com_10324_931com_10324_894col_10324_1209
Santo Domingo, Jaime
Vay, Laura
Camacho, Marcial
Hernández San Miguel, Esther
Fonteriz García, Rosalba Inés
Domínguez Lobatón, María Carmen
Montero Zoccola, María Teresa
Moreno Díaz-Calderón, Alfredo
Álvarez Martín, Javier
2014-09-16T09:52:10Z
2015-09-16T23:40:10Z
2008
European Journal of Neuroscience, 2008, vol. 28, p. 1265-1274
0953-816X
http://uvadoc.uva.es/handle/10324/5977
10.1111/j.1460-9568.2008.06440.x
1265
1274
European Journal of Neuroscience
28
Producción Científica
The secretory granules constitute one of the less well-known compartments in terms of Ca2+ dynamics. They contain large amounts
of total Ca2+, but the free intragranular [Ca2+] ([Ca2+]SG), the mechanisms for Ca2+ uptake and release from the granules and their
physiological significance regarding exocytosis are still matters of debate. We used in the present work an aequorin chimera targeted
to the granules to investigate [Ca2+]SG homeostasis in bovine adrenal chromaffin cells. We found that most of the intracellular
aequorin chimera is present in a compartment with 50–100 lm Ca2+. Ca2+ accumulation into this compartment takes place mainly
through an ATP-dependent mechanism, namely, a thapsigargin-sensitive Ca2+-ATPase. In addition, fast Ca2+ release was observed
in permeabilized cells after addition of inositol 1,4,5-trisphosphate (InsP3) or caffeine, suggesting the presence of InsP3 and
ryanodine receptors in the vesicular membrane. Stimulation of intact cells with the InsP3-producing agonist histamine or with caffeine
also induced Ca2+ release from the vesicles, whereas acetylcholine or high-[K+] depolarization induced biphasic changes in vesicular
[Ca2+], suggesting heterogeneous responses of different vesicle populations, some of them releasing and some taking up Ca2+
during stimulation. In conclusion, our data show that chromaffin cell secretory granules have the machinery required for rapid uptake
and release of Ca2+, and this strongly supports the hypothesis that granular Ca2+ may contribute to its own secretion.
2015-09-16
application/pdf
eng
Federation of European Neuroscience Societies and Blackwell Publishing Ltd
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
Attribution-NonCommercial-NoDerivatives 4.0 International
Médula espinal - Calcio
Calcium dynamics in bovine adrenal medulla chromaffin cell secretory granules
info:eu-repo/semantics/article
SI