2024-03-29T09:52:58Zhttps://uvadoc.uva.es/oai/requestoai:uvadoc.uva.es:10324/68452021-06-23T09:52:02Zcom_10324_1134com_10324_931com_10324_894col_10324_1213
Dinger, Bruce
González, Constancio
Yoshizaki, Katsuaki
Fidone, Salvatore
2014-10-30T13:15:55Z
2014-10-30T13:15:55Z
1985
Brain Research, 1985, vol. 339, p. 295-304
0006-8993
http://uvadoc.uva.es/handle/10324/6845
295
304
Brain Research
339
Producción Científica
Acetylcholine and nicotinic agents excite cat carotid body chemoreceptors and modify their response to natural stimuli. The present
experiments utilized [125I]a-bungarotoxin ([125I]a-BGT) to localize within the chemosensory tissue the possible sites of action of exogenous
and endogenous nicotinic cholinergic substances. In vitro equilibrium binding studies of intact carotid bodies determined a K d of
5.57 nM and a Bma x of 9.21 pmol/g of tissue. Chronic section (12-15 days) of the carotid sinus nerve (CSN) did not change the amount
of displaceable toxin binding. In contrast, the specific binding was reduced by 46% following removal of the superior cervical ganglion.
Light microscope autoradiography of normal, CSN-denervated and sympathectomized carotid bodies revealed displaceable binding
sites concentrated in lobules of type I and type II cells. Treatment of carotid bodies with 50 nM a-BGT in vitro reduced by 50% the
release of [3H]dopamine (synthesized from [3H]tyrosine) caused by hypoxia or nicotine, and also significantly reduced the stimulus-.
evoked discharges recorded from the CSN. The data suggest (1) an absence of ct-BGT binding sites on the afferent terminals of the
CSN and (2) that nicotinic receptors located within parenchymal cell lobules may modulate the release of catecholamines from these cells.
application/pdf
eng
Elsevier
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
Attribution-NonCommercial-NoDerivatives 4.0 International
Neurofisiología
Localization and Function of Cat Carotid Body Nicotinic Receptors
info:eu-repo/semantics/article
SI