2024-03-28T15:26:02Zhttps://uvadoc.uva.es/oai/requestoai:uvadoc.uva.es:10324/247632021-06-24T07:26:56Zcom_10324_22821com_10324_954com_10324_894col_10324_22822
Cross-linking of a biopolymer-peptide co-assembling system
Inostroza Brito, Karla E.
Collin, Estelle
Poliniewicz, Anna
Elsharkawy, Sherif
Rice, Alistair
Río Hernández, Armando E. del
Xiao, Xin
Rodríguez Cabello, José Carlos
Mata, Álvaro
Producción Científica
The ability to guide molecular self-assembly at the nanoscale into complex macroscopic structures could enable the development of functional synthetic materials that exhibit properties of natural tissues such as hierarchy, adaptability, and self-healing. However, the stability and structural integrity of these kinds of materials remains a challenge for many practical applications. We have recently developed a dynamic biopolymer-peptide co-assembly system with the capacity to grow and undergo morphogenesis into complex shapes. Here we explored the potential of different synthetic (succinimidyl carboxymethyl ester, poly (ethylene glycol) ether tetrasuccinimidyl glutarate and glutaraldehyde) and natural (genipin) cross-linking agents to stabilize membranes made from these biopolymer-peptide co-assemblies. We investigated the cross-linking efficiency, resistance to enzymatic degradation, and mechanical properties of the different cross-linked membranes. We also compared their biocompatibility by assessing the metabolic activity and morphology of adipose-derived stem cells (ADSC) cultured on the different membranes. While all cross-linkers successfully stabilized the system under physiological conditions, membranes cross-linked with genipin exhibited better resistance in physiological environments, improved stability under enzymatic degradation, and a higher degree of in vitro cytocompatibility compared to the other cross-linking agents. The results demonstrated that genipin is an attractive candidate to provide functional structural stability to complex self-assembling structures for potential tissue engineering or in vitro model applications.
Ministerio de Economía, Industria y Competitividad (Project MAT2013-42473-R and MAT2015-68901R)
Junta de Castilla y León (programa de apoyo a proyectos de investigación – Ref. VA244U13, VA313U14 and VA015U1
2017-07-27T11:35:57Z
2017-07-27T11:35:57Z
2017
info:eu-repo/semantics/article
Acta Biomaterialia Available online 18 May 2017
http://uvadoc.uva.es/handle/10324/24763
10.1016/j.actbio.2017.05.043
eng
http://www.sciencedirect.com/science/article/pii/S1742706117303331
info:eu-repo/grantAgreement/EC/H2020/646075
Attribution-NonCommercial-NoDerivatives 4.0 International
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
application/pdf
Elsevier
https://uvadoc.uva.es/bitstream/10324/24763/6/Cross-linking.pdf.jpg
Hispana
TEXT
http://creativecommons.org/licenses/by-nc-nd/4.0/
UVaDOC. Repositorio Documental de la Universidad de Valladolid
http://uvadoc.uva.es/handle/10324/24763