2024-03-28T14:46:01Zhttps://uvadoc.uva.es/oai/requestoai:uvadoc.uva.es:10324/351602021-06-24T07:48:59Zcom_10324_35294com_10324_952com_10324_894com_10324_1173com_10324_931com_10324_31059com_10324_954col_10324_35295col_10324_1371col_10324_31061
Multi-production of high added market value metabolites from diluted methane emissions via methanotrophic extremophiles
Cantera Ruiz de Pellón, Sara
Sánchez Andrea, Irene
Lebrero Fernández, Raquel
García Encina, Pedro Antonio
Stams, Alfons J.M.
Muñoz Torre, Raúl
Biorefinería CH4
CH4-biorefinery
Metano
Methane
Producción Científica
This study constitutes the first-proof-of-concept of a methane biorefinery based on the multi-production of high profit margin substances (ectoine, hydroxyectoine, polyhydroxyalkanoates (PHAs) and exopolysaccharides (EPS)) using methane as the sole carbon and energy source. Two bubble column bioreactors were operated under different magnesium concentrations (0.2, 0.02 and 0.002 g L−1) to validate and optimize this innovative strategy for valorization of CH4 emissions. High Mg2+ concentrations promoted the accumulation of ectoine (79.7–94.2 mg g biomass−1), together with high hydroxyectoine yields (up to 13 mg g biomass−1) and EPS concentrations (up to 2.6 g L culture broth−1). Unfortunately, PHA synthesis was almost negligible (14.3 mg L−1) and only found at the lowest Mg2+ concentration tested. Halomonas, Marinobacter, Methylophaga and Methylomicrobium, previously described as ectoine producers, were dominant in both bioreactors, Methylomicrobium being the only described methanotroph. This study encourages further research on CH4 biorefineries capable of creating value out of GHG mitigation.
Ministerio de Economía, Industria y Competitividad (Project CTM2015-70442-RNOVEDAR)
Junta de Castilla y León (programa de apoyo a proyectos de investigación – Ref. E-47-2014-0140696UIC71)
2019-03-21T09:27:07Z
2019-03-21T09:27:07Z
2018
info:eu-repo/semantics/article
Bioresource Technology, 2018, Volume 267, Pages 401-407
0960-8524
http://uvadoc.uva.es/handle/10324/35160
https://doi.org/10.1016/j.biortech.2018.07.057
eng
https://www.sciencedirect.com/science/article/pii/S0960852418309544
Attribution-NonCommercial-NoDerivatives 4.0 International
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
application/pdf
Elsevier
https://uvadoc.uva.es/bitstream/10324/35160/6/Multi-production-high-market-Preprint.pdf.jpg
Hispana
TEXT
http://creativecommons.org/licenses/by-nc-nd/4.0/
UVaDOC. Repositorio Documental de la Universidad de Valladolid
http://uvadoc.uva.es/handle/10324/35160