2024-03-29T05:05:05Zhttps://uvadoc.uva.es/oai/requestoai:uvadoc.uva.es:10324/450702021-06-24T07:16:59Zcom_10324_44585com_10324_954com_10324_894col_10324_44586
Rapid changes in anterior pituitary cell phenotypes in male and female mice after acute cold stress
Senovilla González, Laura
Núñez Llorente, Lucía
Villalobos Jorge, Carlos
García-Sancho Martín, Francisco Javier
Pituitary Cells
Células pituirarias
Phenotyes
Fenotipos
Mice
Ratones
Cold stress
Estrés por frío
Producción Científica
The anterior pituitary (AP) is made of five different cell types. The relative abundance and phenotype of AP cells may change in different physiological situations as an expression of pituitary plasticity. Here, we analyze in detail the phenotype of mouse corticotropes and the effects of acute cold stress on AP cell populations. The hormone content and the expression of hypothalamic-releasing hormone (HRH) receptors in all the five AP cell types were studied in the male and female mice at rest and after a 30-min cold stress. Expression of HRH receptors was evidenced by imaging the single-cell cytosolic Ca2+ responses in fura-2-loaded cells. Hormone contents were studied by multiple, simultaneous immunofluorescence of all the five hormones. Corticotropes displayed a striking sexual dimorphism, even in the resting condition. Male corticotropes showed the orthodox phenotype. They were monohormonal, storing only ACTH, and monoreceptorial, responding only to CRH. In contrast, female corticotropes were made of about equal parts of orthodox cells and multifunctional cells, which co-stored additional AP hormones and expressed additional HRH receptors. Cold stress did not modify the number of ACTH containing cells, but, according to immunostaining, it increased the relative abundance of other AP cell types at the expense of the pool of cells storing no hormones. Cold stress also modified the response to CRH and other HRHs. Most of these phenotypical changes presented a strong sexual dimorphism. These results indicate that pituitary plasticity is even larger than previously thought.
Fondo de Investigaciones Sanitarias (grant FIS03/1231)
Ministerio de Ciencia, Innovación y Universidades (grants BFU-2004-02765/BFI, BFU2005-02078 and BFU2007-60157)
2021-01-19T10:10:03Z
2021-01-19T10:10:03Z
2008
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Endocrinology, 2008, vol. 149, n. 5. p. 2159–2167
1945-7170
http://uvadoc.uva.es/handle/10324/45070
10.1210/en.2007-1030
eng
https://academic.oup.com/endo/article/149/5/2159/2454880
Attribution-NonCommercial-NoDerivs 3.0 Unported
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/3.0/
© 2008 Oxford University Press
application/pdf
Oxford University Press
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Hispana
TEXT
http://creativecommons.org/licenses/by-nc-nd/3.0/
UVaDOC. Repositorio Documental de la Universidad de Valladolid
http://uvadoc.uva.es/handle/10324/45070