2024-03-29T14:40:39Zhttps://uvadoc.uva.es/oai/requestoai:uvadoc.uva.es:10324/524552022-03-15T21:48:06Zcom_10324_31661com_10324_952com_10324_894col_10324_31662
Phenolic Compounds Extraction of Arbutus unedo L.: Process Intensification by Microwave Pretreatment
Alexandre, Agostinho
Matias, Ana A.
Bronze, Maria Rosario
Cocero Alonso, María José
Mato Chaín, Rafael Bartolomé
Arbutus unedo
Phenolic compounds
Compuestos fenólicos
Producción Científica
Arbutus unedo L., commonly known as the strawberry-tree fruit, is an endemic species of the Mediterranean flora. Microwave extraction technology has been considered as a fast and “green” method for the production of extracts rich in bioactive compounds, although the energy consumption is high. To overcome this bottleneck, microwave was used as a pretreatment procedure in short time periods. This technique promotes the burst of intracellular vacuoles leading to an increase in the lixiviation of phenolic compounds. Different approaches were tested, namely a solvent-free irradiation (SFI), a solvent-assisted irradiation (SAI) and a pressurized solvent-assisted irradiation (PSAI). After irradiation, a solid–liquid extraction procedure was performed using a mixture of water and ethanol. A kinetic evaluation of the total phenolic content (TPC) was performed using the Folin–Ciocalteu method. For the total anthocyanin content, a UV-spectrophotometric method was used. HPLC-UV and LC-MS were used for TPC and identification of present compounds. Microwave irradiation led to an increase in TPC of extracts after SAI (52%) and PSAI (66%) along with a reduction in time of extraction from 30 min to less than 2 min. The anthocyanin content also increased by 66% for the SAI and PSAI extractions.
Fundação para Ciência e Tecnologia (fellowship SFRH/BD/52532/2014)
Fundação para a Ciência e Tecnologia/Ministério da Educação e Ciência - Fondo Europeo de Desarrollo Regional (partnership PT2020)
Portuguese National Mass Spectrometry Network (contracts RNEM—REDE/1518/REM/2005, ROTEIRO/0028/2013 and LISBOA-01-0145-FEDER-022125)
Marie Curie Industry - Academia Partnerships and Pathways (project FP7-PEOPLE-2013-IAPP-612208)
2022-03-15T10:08:58Z
2022-03-15T10:08:58Z
2020
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Processes, 2020, vol. 8, n. 3, 298
2227-9717
https://uvadoc.uva.es/handle/10324/52455
10.3390/pr8030298
eng
https://www.mdpi.com/2227-9717/8/3/298
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
© 2020 The Authors
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UVaDOC. Repositorio Documental de la Universidad de Valladolid
https://uvadoc.uva.es/handle/10324/52455