2024-03-28T16:03:48Zhttps://uvadoc.uva.es/oai/requestoai:uvadoc.uva.es:10324/527402022-04-19T20:47:30Zcom_10324_1173com_10324_931com_10324_894col_10324_1371
Optimization of nitrogen feeding strategies for improving polyhydroxybutyrate production from biogas by Methylocystis parvus OBBP in a stirred tank reactor
Rodríguez Muñoz, Yadira
García, Silvia María
Pérez Fernández, Rebeca
Lebrero Fernández, Raquel
Muñoz Torre, Raúl
Bioplastics
Bioplásticos
Biorefinery
Biorrefinería
Methylocystis parvus
Producción Científica
The design of efficient cultivation strategies to produce bioplastics from biogas is crucial for the implementation of this biorefinery process. In this work, biogas-based polyhydroxybutyrate (PHB) production and CH4 biodegradation performance was investigated for the first time in a stirred tank bioreactor inoculated with Methylocystis parvus OBBP. Decreasing nitrogen loading rates in continuous mode and alternating feast:famine regimes of 24 h-cycles, and alternating feast:famine regimes of 24 h:24 h and 24 h:48 h were tested. Continuous N feeding did not support an effective PHB production despite the occurrence of nitrogen limiting conditions. Feast-famine cycles of 24 h:24 h (with 50% stoichiometric nitrogen supply) supported the maximum PHB production (20 g-PHB m−3 d−1) without compromising the CH4-elimination capacity (25 g m−3 h−1) of the system. Feast:famine ratios ≤1:2 entailed the deterioration of process performance at stoichiometric nitrogen inputs ≤60%.
Ministerio de Ciencia, Innovación y Universidades (grant BES-2016-077160)
Ministerio de Ciencia, Innovación y Universidades (project CTM2015-70442-R)
Junta de Castilla y León (projects UIC 071 and CLU 2017–09)
2022-03-30T11:41:58Z
2022-03-30T11:41:58Z
2022
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Chemosphere, 2022, vol. 299, 134443
0045-6535
https://uvadoc.uva.es/handle/10324/52740
10.1016/j.chemosphere.2022.134443
eng
https://www.sciencedirect.com/science/article/pii/S0045653522009365#ack0010
Atribución 4.0 Internacional
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
© 2022 Elsevier
application/pdf
Elsevier
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Hispana
TEXT
http://creativecommons.org/licenses/by/4.0/
UVaDOC. Repositorio Documental de la Universidad de Valladolid
https://uvadoc.uva.es/handle/10324/52740