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oai:uvadoc.uva.es:10324/636562025-02-18T11:25:51Zcom_10324_28542com_10324_952com_10324_894col_10324_28543

Development and validation of an analytical methodology based on solvent extraction and gas chromatography for determining pesticides in royal jelly and propolis De La Fuente Ballesteros, Adrián Jano, Ana Bernal del Nozal, José Ares Sacristán, Ana María Alimentos - Análisis Pesticidas Food analysis Propolis Pesticides Análisis de alimentos Propóleos Pesticidas 2301 Química Analítica Producción Científica We propose a new analytical methodology to determine seven pesticides (atrazine, chlorpyrifos, chlorfenvinphos, α-endosulfan, bromopropylate, coumaphos, and τ-fluvalinate) in royal jelly and propolis products using gas chromatography-mass spectrometry. Sample treatment, with minor modifications for propolis, consisted of a solvent extraction with a hexane and isopropanol mixture, and a further clean-up step. Meanwhile, chromatographic analysis (<25 min) was performed in a DB-5MS column under programmed temperature conditions. In all cases we validated the method in terms of selectivity, limits of detection (0.1–2.8 μg kg−1) and quantification (0.3–9.2 μg kg−1), linearity, matrix effect (<±20 %), trueness (recoveries between 93 % and 118 %), and precision (relative standard deviation < 11 %). All royal jelly liquid dietary supplements were positive for chlorfenvinphos and, in the case of one of them, for α-endosulfan; chlorfenvinphos was determined in some fresh royal jelly samples, and no pesticide residues were detected in the propolis samples analysed Plan Nacional de Investigación e Innovación Científica y Técnica, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria-INIA-FEDER (grant number RTA2017-00004-C02-02) 2023-12-15T12:54:56Z 2023-12-15T12:54:56Z 2024 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Food Chemistry, 2024, vol. 437, Part 2, 137911 0308-8146 https://uvadoc.uva.es/handle/10324/63656 10.1016/j.foodchem.2023.137911 137911 Food Chemistry 437 eng https://www.sciencedirect.com/science/article/pii/S0308814623025293?via%3Dihub Attribution-NonCommercial-NoDerivatives 4.0 Internacional info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/4.0/ © 2023 The Authors application/pdf Elsevier https://uvadoc.uva.es/bitstream/10324/63656/4/Development-and-validation.pdf.jpg Hispana TEXT http://creativecommons.org/licenses/by-nc-nd/4.0/ UVaDOC. Repositorio Documental de la Universidad de Valladolid https://uvadoc.uva.es/handle/10324/63656