2024-03-28T12:25:45Zhttps://uvadoc.uva.es/oai/requestoai:uvadoc.uva.es:10324/217532021-06-23T11:48:36Zcom_10324_1185com_10324_931com_10324_894col_10324_1346
Hybrid elastin-like recombinamer-fibrin gels: physical characterization and in vitro evaluation for cardiovascular tissue engineering applications
González de Torre, Israel
Weber, Miriam T.
Quintanilla Sierra, Luis
Alonso Rodrigo, Matilde
Jockenhoevel, Stefan
Rodríguez Cabello, José Carlos
Mela, Petra
Colágeno
Producción Científica
In the field of tissue engineering, the properties of the scaffolds are of crucial importance for the success of the application. Hybrid materials combine the properties of the different components that constitute them. In this study hybrid gels of Elastin-Like Recombinamer (ELR) and fibrin were prepared with a range of polymer concentrations and ELR-to-fibrin ratios. The correlation between SEM micrographs, porosities, swelling ratios and rheological properties was discussed and a poroelastic mechanism was suggested to explain the mechanical behavior of the hybrid gels. Applicability as scaffold materials for cardiovascular tissue engineering was shown by the realization of cell-laden matrixes which supported the synthesis of collagens as revealed by immunohistochemical analysis. As a proof of concept, a tissue-engineered heart valve was fabricated by injection moulding and cultivated in a bioreactor for 3 weeks under dynamic con- ditions. Tissue analysis revealed the production of collagen I and III, fundamental proteins for cardio- vascular constructs.
2016-12-15
2016-12-15
2016
info:eu-repo/semantics/article
Biomaterials Science, 2016 vol. 4, 1361-1370
2047-4830
http://uvadoc.uva.es/handle/10324/21753
10.1039/C6BM00300A
1361
4
1370
Biomaterials Science
eng
http://pubs.rsc.org/
info:eu-repo/grantAgreement/EC/H2020/263363
info:eu-repo/grantAgreement/EC/H2020/278557
info:eu-repo/grantAgreement/EC/H2020/317304
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
Attribution-NonCommercial-NoDerivatives 4.0 International
Royal Society of Chemistry