2024-03-28T11:20:45Zhttps://uvadoc.uva.es/oai/requestoai:uvadoc.uva.es:10324/42022021-09-13T09:29:16Zcom_10324_1181com_10324_931com_10324_894col_10324_1387
00925njm 22002777a 4500
dc
León, Alberto José
author
Garrote Adrados, José Antonio
author
Blanco Quirós, Alfredo
author
Calvo Romero, María del Carmen
author
Fernández Salazar, Luis Ignacio
author
Villar, A. del
author
Barrera, Asterio
author
Arranz Sanz, Eduardo
author
2006
Dietary gluten induces an early response in the intestine of coeliac disease
patients (CD), within a few hours, and this is driven by high levels of proinflammatory
cytokines, including IFNg and IL-15, as has been thoroughly
shown by gluten stimulation of biopsy explants. Our aim was to identify the
immune mediators involved in the long-standing inflammation in untreated
CD patients at diagnosis. mRNA and protein levels of TNFa, IL-12(p35),
IL-12(p40), IL-15, IL-18 and IL-23(p19) were quantified in biopsies from
active CD patients, CD patients on a gluten-free diet (GFD), healthy controls,
and patients with non-CD inflammation and mild histological changes in the
intestine. Biopsies from CD patients on a GFD were also stimulated in vitro
with gliadin, and protein expression of IL-15 and IL-18 was analysed. Levels of
IL-12 and IL-23 mRNA are nearly absent, and TNFa levels remain unchanged
among different groups. Both the active and inactive forms of IL-18 protein
have been found in all samples from active CD, and protein expression was
only localized within the crypts. Levels of IL-15 mRNA remain unchanged,
and protein expression, localized within the lamina propria, is found in a
small number of samples. In vitro stimulation with gluten induces the expression
of IL-15 and IL-18. In active CD, the early response following gluten
intake characterized by high IFNg levels is driven by IL-18, and probably
IL-15, and this alternates with periods of long-standing inflammation with
moderate IFNg levels, maintained by IL-18 alone.
Clinical and Experimental Immunology, 146: 479-485
0009-9104
http://uvadoc.uva.es/handle/10324/4202
10.111/j.1365-2249.2006.03239.X
479
146
485
Clinical and Experimental Immunology
Enfermedad celíaca
Intestinos - Enfermedades
Interleukin 18 maintains a long-standing inflammation in coeliac disease patients