2024-03-28T08:47:44Zhttps://uvadoc.uva.es/oai/requestoai:uvadoc.uva.es:10324/425902021-11-03T13:27:55Zcom_10324_1134com_10324_931com_10324_894col_10324_1213
00925njm 22002777a 4500
dc
González-Vallinas Garrachón, Margarita
author
Molina, Susana
author
Vicente, Gonzalo
author
Zarza, Virginia
author
Martín Hernández, Roberto
author
García Risco, Mónica R.
author
Fornari, Tiziana
author
Reglero, Guillermo
author
Ramírez de Molina, Ana
author
2014
Colorectal and pancreatic cancers remain important contributors to cancer mortality burden and, therefore, new
therapeutic approaches are urgently needed. Rosemary (Rosmarinus officinalis L.) extracts and its components have been
reported as natural potent antiproliferative agents against cancer cells. However, to potentially apply rosemary as a
complementary approach for cancer therapy, additional information regarding the most effective composition, its
antitumor effect in vivo and its main molecular mediators is still needed. In this work, five carnosic acid-rich supercritical
rosemary extracts with different chemical compositions have been assayed for their antitumor activity both in vivo (in nude
mice) and in vitro against colon and pancreatic cancer cells. We found that the antitumor effect of carnosic acid together
with carnosol was higher than the sum of their effects separately, which supports the use of the rosemary extract as a
whole. In addition, gene and microRNA expression analyses have been performed to ascertain its antitumor mechanism,
revealing that up-regulation of the metabolic-related gene GCNT3 and down-regulation of its potential epigenetic
modulator miR-15b correlate with the antitumor effect of rosemary. Moreover, plasmatic miR-15b down-regulation was
detected after in vivo treatment with rosemary. Our results support the use of carnosic acid-rich rosemary extract as a
complementary approach in colon and pancreatic cancer and indicate that GCNT3 expression may be involved in its
antitumor mechanism and that miR-15b might be used as a non-invasive biomarker to monitor rosemary anticancer effect.
PLoS ONE, 2014, vol 9, n. 6, e98556
1932-6203
http://uvadoc.uva.es/handle/10324/42590
10.1371/journal.pone.0098556
e98556
6
PLoS ONE
9
1932-6203
Expression of MicroRNA-15b and the glycosyltransferase GCNT3 correlates with antitumor efficacy of rosemary diterpenes in colon and pancreatic cancer