2026-05-18T15:20:07Zhttps://uvadoc.uva.es/oai/request

oai:uvadoc.uva.es:10324/556022025-02-13T10:09:32Zcom_10324_40357com_10324_952com_10324_894col_10324_40358

00925njm 22002777a 4500 dc Katsinas, Nicolaos author Gehlsen, Uta author García Posadas, Laura author Rodríguez Rojo, Soraya author Steven, Philipp author González García, María Jesús author Enriquez De Salamanca Aladro, Amalia author 2022 Dry eye (DED) is a prevalent disease with immune-mediated inflammation as the principal pathophysiological etiology. Olive pomace, the major by-product of the olive oil industry, is rich in high-value polyphenols. Their anti-inflammatory and immunomodulatory activities were determined on human CD4+ T cells (hTCD4+) and in a DED animal model. The viability of hTCD4+ cells isolated from peripheral blood and activated with phytohemagglutinin-M was evaluated after treatment for 48 h with an olive pomace extract (OPT3, 0.10–0.40 mg/mL) and its major compound, hydroxytyrosol (25–100 μM). Regarding the DED animal model, 100 μM hydroxytyrosol, 0.20 mg/mL OPT3, or vehicle (borate buffer) were topically administered to 14 days-desiccating stress-exposed (constant airflow/scopolamine administration) C57BL/6 mice. Tear volume, corneal fluorescein staining (CFS), CD4+, and CD8+ T cell count in lymph nodes (flow cytometry), and IP-10 and TNF-α gene expression (qRT-PCR) in the cornea, conjunctiva, and lacrimal glands were evaluated. OPT3 (0.2–0.4 mg/mL) and hydroxytyrosol (100 μM) significantly reduced hTCD4+ proliferation. In mice, both treatments reduced lacrimal gland IP-10 gene expression. OPT3 also decreased CFS, and conjunctival IP-10 and corneal TNF-α gene expression. In lymph nodes, hydroxytyrosol reduced CD3+, OPT3, and CD8+ count. Thus, a high-value application as a promising DED protection was proposed for olive pomace. Journal of Clinical Medicine, 2022, Vol. 11, Nº. 16, 17 pp. 2077-0383 https://uvadoc.uva.es/handle/10324/55602 10.3390/jcm11164703 1 16 17 Journal of Clinical Medicine 11 2077-0383 Ojo - Enfermedades y defectos - Tratamiento Síndrome de ojo seco Aceite de oliva Aceite de oliva - Análisis Olive pomace phenolic compounds: from an agro-industrial by-product to a promising ocular surface protection for dry eye disease