2024-03-28T10:55:41Zhttps://uvadoc.uva.es/oai/requestoai:uvadoc.uva.es:10324/584582023-02-02T20:00:24Zcom_10324_1134com_10324_931com_10324_894col_10324_1213
00925njm 22002777a 4500
dc
Álvarez Illera, María Pilar
author
García Casas, Paloma
author
Fonteriz García, Rosalba Inés
author
Montero Zoccola, María Teresa
author
Álvarez Martín, Javier
author
2020
Mitochondrial [Ca2+] plays an important role in the regulation of mitochondrial function, controlling ATP production and apoptosis triggered by mitochondrial Ca2+ overload. This regulation depends on Ca2+ entry into the mitochondria during cell activation processes, which is thought to occur through the mitochondrial Ca2+ uniporter (MCU). Here, we have studied the mitochondrial Ca2+ dynamics in control and MCU-defective C. elegans worms in vivo, by using worms expressing mitochondrially-targeted YC3.60 yellow cameleon in pharynx muscle. Our data show that the small mitochondrial Ca2+ oscillations that occur during normal physiological activity of the pharynx were very similar in both control and MCU-defective worms, except for some kinetic differences that could mostly be explained by changes in neuronal stimulation of the pharynx. However, direct pharynx muscle stimulation with carbachol triggered a large and prolonged increase in mitochondrial [Ca2+] that was much larger in control worms than in MCU-defective worms. This suggests that MCU is necessary for the fast mitochondrial Ca2+ uptake induced by large cell stimulations. However, low-amplitude mitochondrial Ca2+ oscillations occurring under more physiological conditions are independent of the MCU and use a different Ca2+ pathway.
International Journal of Molecular Sciences, 2020, Vol. 21, Nº.22, 8622
1661-6596
https://uvadoc.uva.es/handle/10324/58458
10.3390/ijms21228622
8622
22
International Journal of Molecular Sciences
21
1422-0067
Mitocondrias
Genética médica
Calcio - Metabolismo
Mitochondrial Ca2+ dynamics in MCU knockout C. elegans worms