2026-04-17T02:28:54Zhttps://uvadoc.uva.es/oai/request
oai:uvadoc.uva.es:10324/491562021-11-17T12:38:51Zcom_10324_31661com_10324_952com_10324_894col_10324_31662
UVaDOC author Salvo Comino, Coral author Martín Pedrosa, Fernando author García Cabezón, Ana Cristina author Rodríguez Méndez, María Luz 2021-10-19T08:01:47Z 2021-10-19T08:01:47Z 2021 Sensors, 2021, vol. 21, n. 3, 899 1424-8220 https://uvadoc.uva.es/handle/10324/49156 10.3390/s21030899 899 3 Sensors 21 1424-8220 The integration of nanomaterials as electron mediators in electrochemical biosensors is taking on an essential role. Due to their high surface-to-volume ratio and high conductivity, metallic nanowires are an interesting option. In this paper, silver nanowires (AgNWs) were exploited to design a novel catechol electrochemical biosensor, and the benefits of increasing the aspect ratio of the electron mediator (nanowires vs. nanoparticles) were analyzed. Atomic force microscopy (AFM) studies have shown a homogeneous distribution of the enzyme along the silver nanowires, maximizing the contact surface. The large contact area promotes electron transfer between the enzyme and the electrode surface, resulting in a Limit of Detection (LOD) of 2.7 × 10−6 M for tyrosinase immobilized onto AgNWs (AgNWs-Tyr), which is one order of magnitude lower than the LOD of 3.2 × 10−5 M) obtained using tyrosinase immobilized onto silver nanoparticles (AgNPs-Tyr). The calculated KM constant was 122 mM. The simultaneous use of electrochemistry and AFM has demonstrated a limited electrochemical fouling that facilitates stable and reproducible detection. Finally, the biosensor showed excellent anti-interference characteristics toward the main phenols present in wines including vanillin, pyrogallol, quercetin and catechin. The biosensor was able to successfully detect the presence of catechol in real wine samples. These results make AgNWs promising elements in nanowired biosensors for the sensitive, stable and rapid voltammetric detection of phenols in real applications. eng Atribución 4.0 Internacional Silver nanowires as electron transfer mediators in electrochemical catechol biosensors info:eu-repo/semantics/article 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URL https://uvadoc.uva.es/bitstream/10324/49156/1/Silver-nanowires-as-electron.pdf File MD5 12e125be8ce5f910d1c2e547e66d2248 1856110 application/pdf Silver-nanowires-as-electron.pdf