2026-06-10T06:11:56Zhttps://uvadoc.uva.es/oai/requestoai:uvadoc.uva.es:10324/558552025-03-03T08:10:28Zcom_10324_32522com_10324_952com_10324_894col_10324_32523
UVaDOC
author
Lebrero Fernández, Patricia
author
Astudillo del Valle, Alma María
author
Rubio, Julio M.
author
Fernández-Caballero Palomeque, Lidia
author
Kokotos, George
author
Balboa García, María Ángeles
author
Balsinde Rodríguez, Jesús
2022-10-06T11:09:36Z
2022-10-06T11:09:36Z
2019
Cells, 2019, vol. 8, n. 8, 799
2073-4409
https://uvadoc.uva.es/handle/10324/55855
10.3390/cells8080799
Availability of free arachidonic acid (AA) constitutes a rate limiting factor for cellular eicosanoid synthesis. AA distributes differentially across membrane phospholipids, which is largely due to the action of coenzyme A-independent transacylase (CoA-IT), an enzyme that moves the fatty acid primarily from diacyl phospholipid species to ether-containing species, particularly the ethanolamine plasmalogens. In this work, we examined the dependence of AA remodeling on plasmalogen content using the murine macrophage cell line RAW264.7 and its plasmalogen-deficient variants RAW.12 and RAW.108. All three strains remodeled AA between phospholipids with similar magnitude and kinetics, thus demonstrating that cellular plasmalogen content does not influence the process. Cell stimulation with yeast-derived zymosan also had no effect on AA remodeling, but incubating the cells in AA-rich media markedly slowed down the process. Further, knockdown of cytosolic-group IVC phospholipase A2γ (cPLA2γ) by RNA silencing significantly reduced AA remodeling, while inhibition of other major phospholipase A2 forms such as cytosolic phospholipase A2α, calcium-independent phospholipase A2β, or secreted phospholipase A2 had no effect. These results uncover new regulatory features of CoA-IT-mediated transacylation reactions in cellular AA homeostasis and suggest a hitherto unrecognized role for cPLA2γ in maintaining membrane phospholipid composition via regulation of AA remodeling.
eng
Atribución 4.0 Internacional
Cellular plasmalogen content does not influence arachidonic acid levels or distribution in macrophages: A role for cytosolic phospholipase A2γ in phospholipid remodeling
info:eu-repo/semantics/article
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IApwb3IgaW5jdW1wbGltaWVudG8gZGUgY3VhbHF1aWVyYSBkZSBsYXMgb2JsaWdhY2lvbmVzIGRlcml2YWRhcyBkZWwgQWN1ZXJkbywgcG9yIHZvbHVudGFkIGV4cHJlc2EgZGVsIEF1dG9yCkVuICBwcnVlYmEgZGUgY29uZm9ybWlkYWQsIGxhcyBwYXJ0ZXMgYWNlcHRhbiBlbCBwcmVzZW50ZSBBY3VlcmRvCgoKCgo=
URL
https://uvadoc.uva.es/bitstream/10324/55855/1/Cellular-plasmalogen-content.pdf
File
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42049b74b315ee14ab9affc8a3a228e6
2029128
application/pdf
Cellular-plasmalogen-content.pdf