2026-06-11T00:00:29Zhttps://uvadoc.uva.es/oai/request
oai:uvadoc.uva.es:10324/660782024-12-17T10:58:32Zcom_10324_1134com_10324_931com_10324_894com_10324_32522com_10324_952col_10324_1213col_10324_32523
UVaDOC author Srinivasan, Sumithra Yasaswini author Álvarez Illera, María Pilar author Kukhtar, Dmytro author Benseny-Cases, Núria author Cerón, Julián author Álvarez Martín, Javier author Fonteriz García, Rosalba Inés author Montero Zoccola, María Teresa author Laromaine, Anna 2024-02-09T11:43:38Z 2024-02-09T11:43:38Z 2023 ACS Nano, 2023, vol. 17, n. 17, p. 17273−17284 1936-0851 https://uvadoc.uva.es/handle/10324/66078 10.1021/acsnano.3c05245 17273 17 17284 ACS Nano 17 1936-086X Experimental studies and clinical trials of nanoparticles for treating diseases are increasing continuously. However, the reach to the market does not correlate with these efforts due to the enormous cost, several years of development, and off-target effects like cardiotoxicity. Multicellular organisms such as the Caenorhabditis elegans (C. elegans) can bridge the gap between in vitro and vertebrate testing as they can provide extensive information on systemic toxicity and specific harmful effects through facile experimentation following 3R EU directives on animal use. Since the nematodes’ pharynx shares similarities with the human heart, we assessed the general and pharyngeal effects of drugs and polypyrrole nanoparticles (Ppy NPs) using C. elegans. The evaluation of FDA-approved drugs, such as Propranolol and Racepinephrine reproduced the arrhythmic behavior reported in humans and supported the use of this small animal model. Consequently, Ppy NPs were evaluated due to their research interest in cardiac arrhythmia treatments. The NPs’ biocompatibility was confirmed by assessing survival, growth and development, reproduction, and transgenerational toxicity in C. elegans. Interestingly, the NPs increased the pharyngeal pumping rate of C. elegans in two slow-pumping mutant strains, JD21 and DA464. Moreover, the NPs increased the pumping rate over time, which sustained up to a day post-excretion. By measuring pharyngeal calcium levels, we found that the impact of Ppy NPs on the pumping rate could be mediated through calcium signaling. Thus, evaluating arrhythmic effects in C. elegans offers a simple system to test drugs and nanoparticles, as elucidated through Ppy NPs. eng Attribution-NonCommercial-NoDerivatives 4.0 Internacional Arrhythmic effects evaluated on Caenorhabditis elegans: the case of polypyrrole nanoparticles info:eu-repo/semantics/article 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URL https://uvadoc.uva.es/bitstream/10324/66078/1/2023-ACS%20Nano.pdf File MD5 138f6683fccb328960ac722e7f61754e 8185575 application/pdf 2023-ACS Nano.pdf