2024-03-29T01:19:31Zhttps://uvadoc.uva.es/oai/requestoai:uvadoc.uva.es:10324/49462021-06-23T09:47:37Zcom_10324_1132com_10324_931com_10324_894col_10324_1205
Gato Casado, Ángel Luis
Martín, C.
Alonso Revuelta, María Isabel
Martínez Álvarez, C.
Moro Balbás, José Antonio
2014-06-09T06:56:44Z
2014-06-09T06:56:44Z
2001
Exp. Eye. Res., 2001, 58 p.1-8
0014-4835
http://uvadoc.uva.es/handle/10324/4946
10.1006/exer.2001.1060
1
8
Exp. Eye. Res.
58
Proteoglycans have been implicated in the invagination and formation of various embryonal cavitied
primordia. In this paper the expression of chondroitin sulphate proteoglycan is analysed (CSPG) in the lens
primordium during lens vesicle formation, and demonstrate that this proteoglycan has a speci®c
distribution pattern with regard to invagination and fusion processes in the transformation of placode into
lens vesicle. More speci®cally, CSPG was detached in: (1) the apical surface of lens epithelial cells, where
early CSPG expression was observed in the whole of the lens placode whilst in the vesicle phase it was
restricted to the posterior epithelium; (2) intense CSPG expression in the basal lamina, which remained
constant for the entire period under study; (3) CSPG expression in the intercellular spaces of the lens
primordium epithelium, which increased during the invagination of the primordium and which at the
vesicle stage was more evident in the posterior epithelium; and (4) CSPG expression on the edges of the
lens placode both prior to and during fusion. Treatment with b-D-xyloside causes signi®cant CSPG
depletion in the lens primordium together with severe alterations in the invagination and fusion of the
lens vesicle; this leads to the formation of lens primordia which in some cases remain practically ¯at or
show partial invagination defects or fusion disruption. Similar results were obtained by enzyme digestion
with chondroitinase AC but not with type II heparinase, which indicates that alterations induced by b-Dxyloside
were due to interference in CSPG synthesis. The ®ndings demonstrate that CSPG is a common
component of the lens primordium at the earliest developmental stages during which it undergoes speci®c
modi®cations. It also includes experimental evidence to show that `in vivo' CSPG plays an important role
in the invagination and fusion processes of the lens primordium. # 2001 Academic Press
Key words: lens development; CSPG; b-D-xyloside; extracellular matrix; epithelial invagination
eng
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
Attribution-NonCommercial-NoDerivatives 4.0 International
Embriología
Chondroitin sulphate proteoglycan is involved in lens vesicle morphogenesis in chick embryos
info:eu-repo/semantics/article