2024-03-29T12:53:57Zhttps://uvadoc.uva.es/oai/requestoai:uvadoc.uva.es:10324/403762021-06-24T07:27:49Zcom_10324_22821com_10324_954com_10324_894col_10324_22822
Recombinant AMP/Polypeptide Self-Assembled Monolayers with Synergistic Antimicrobial Properties for Bacterial Strains of Medical Relevance
Acosta Rodríguez, Sergio
Quintanilla Sierra, Luis
Alonso Rodrigo, Matilde
Aparicio, Conrado
Rodríguez Cabello, José Carlos
Antimicrobial peptides
Péptido antimicrobiano
Self-assembled monolayers
Monocapas autoensambladas
Elastin-like recombinamers
Recombinantes tipo elastina
Bacterial strains
Cepas bacterianas
Producción Científica
Nosocomial infections are one of the most frequent causes of indwelling biomedical device failure. In this regard, the use of antibiofilm nanocoatings based on antimicrobial peptides (AMPs) is a promising alternative to prevent multiresistant biofilm infections. However, the limitations of chemical production impede the large-scale development of advanced antimicrobial materials that improve the properties of AMPs. Herein, we present a multifunctional modular design for the recombinant coproduction of self-assembled monolayers (SAMs) based on AMPs and elastin-like recombinamers (ELRs), which combine the antimicrobial properties of a designer AMP, GL13K, and low-fouling activity of an ELR in a synergistic manner. The inclusion of a grafting domain intended for oriented tethering onto surfaces allowed the recombinant polymers to be covalently immobilized onto model gold surfaces. The antibiofilm properties against two of the bacterial strains most frequently responsible for indwelling medical device-associated infections, namely Staphylococcus epidermidis and Staphylococcus aureus, were then evaluated. GL13K peptide was found to provide antibiofilm properties to the surface, with these being synergistically enhanced by the antifouling effect of the ELR. This new design offers a promising tool for the development of advanced AMP-based nanocoatings for medical devices with powerful and enhanced features.
Comisión Europea (project NMP-2014-646075)
Ministerio de Economía, Industria y Competitividad (project PCIN-2015-010 / MAT2015-68901-R / MAT2016-78903-R)
Junta de Castilla y León (project VA317P18)
2020-01-29T08:59:00Z
2020-01-29T08:59:00Z
2019
info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
https://doi.org/10.1021/acsbiomaterials.9b00247
ACS Biomaterials Science & Engineering, 2019, vol. 5, n. 9. p. 4708-4716
2373-9878
http://uvadoc.uva.es/handle/10324/40376
4708
9
4716
ACS Biomaterials Science & Engineering
5
2373-9878
eng
https://pubs.acs.org/doi/abs/10.1021/acsbiomaterials.9b00247
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
© 2019 American Chemical Society
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ACS Publications