2024-03-29T04:46:25Zhttps://uvadoc.uva.es/oai/requestoai:uvadoc.uva.es:10324/217662021-06-23T11:48:55Zcom_10324_1185com_10324_931com_10324_894col_10324_1346
Mineralization and bone regeneration using a bioactive elastin-like recombinamer membrane
Klymov, Alexey
Reza Nejadnik, M.
Alonso Rodrigo, Matilde
Rodríguez Cabello, José Carlos
Walboomers, X. Frank
Mata, Álvaro
Ingeniería Química
In the field of tissue engineering, the properties of the scaffolds are of crucial importance for the success of the application. Hybrid materials combine properties of the different components that constitute them. In this study hybrid gels of elastin-like recombinamer (ELR) and fibrin were prepared with a range on polymer concentrations and ELR-to-fibrin ratios. The correlation between SEM micrographs, porosity, swelling ratio and rheological properties was discussed and a poroelastic mechanism was suggested to explain the mechanical behavior of the hybrid gels. Applicability as scaffold material for cardiovascular tissue engineering was shown by the realization of cell-laden matrixes which supported the synthesis of collagens as revealed by immunohistochemical analysis. As a proof of concept, a tissue-engineered heart valve was fabricated by injection moulding and cultivated in a bioreactor for 3 weeks under dynamic conditions. Tissue analysis revealed production of collagen I and III, fundamental proteins for cardiovascular constructs.
2016-12-15T11:50:18Z
2016-12-15T11:50:18Z
2016-12-15T11:50:18Z
2014
info:eu-repo/semantics/article
Biomaterials, 2014, Septiembre, vol. 35 n. 29 p. 8339-47
0142-9612
http://uvadoc.uva.es/handle/10324/21766
10.1016/j.biomaterials.2014.05.095.
8339
29
8347
Biomaterials
vol. 35
spa
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
Attribution-NonCommercial-NoDerivatives 4.0 International