2024-03-28T15:27:41Zhttps://uvadoc.uva.es/oai/requestoai:uvadoc.uva.es:10324/364312021-11-08T09:33:23Zcom_10324_22821com_10324_954com_10324_894col_10324_22822
Silk-ELR co-recombinamer covered stents obtained by electrospinning
Putzu, M.
Causa, F.
Parente, Manuel
González de Torre, Israel
Rodríguez Cabello, José Carlos
Netti, P.A.
Elastin-like recombinamers
Recombinantes tipo elastina
Tissue engineering
Ingeniería de tejidos
In the field of tissue engineering the choice of materials is of great importance given the possibility
to use biocompatible polymers produced by means of biotechnology. A large number of synthetic
and natural materials have been used to this purpose and processed into scaffolds using
Electrospinning technique. Among materials that could be used for the fabrication of scaffold and
degradable membranes, natural polymers such as collagen, elastin or fibroin offer the possibility
to design structures strictly similar to the extracellular matrix (ECM). Biotechnology and genetic engineering
made possible the advent of a new class of biopolymers called protein-based polymers.
One example is represented by the silk-elastin-proteins that combine the elasticity and resilience of
elastin with the high tensile strength of silk-fibroin and display engineered bioactive sequences. In
this work, we use electrospinning technique to produce a fibrous scaffold made of the corecombinamer
Silk-ELR. Obtained fibres have been characterized from the morphological point of
view. Homogeneity and morphology have been explored using Scanning Electron Microscopy. A
thorough study regarding the influence of Voltage, flow rate and distance have been carried out to
determine the appropriate parameters to obtain the fibrous mats without defects and with a good
distribution of diameters. Cytocompatibility has also been in vitro tested. For the first time we use
the co-recombinamer Silk-ELR for the fabrication of a 2.5 angioplasty balloon coating. This structure
could be useful as a coated scaffold for the regeneration of intima layer of vessels.
2019-06-25T11:52:40Z
2019-06-25T11:52:40Z
2019-06-25T11:52:40Z
2019
info:eu-repo/semantics/article
Regenerative Biomaterials, 2019, vol. 6, n. 1. p. 21–28
2056-3426
http://uvadoc.uva.es/handle/10324/36431
https://doi.org/10.1093/rb/rby022
eng
https://academic.oup.com/rb/article/6/1/21/5146764
info:eu-repo/grantAgreement/EC/FP7/278557
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
© 2019 Oxford University Press
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Oxford University Press