2024-03-29T10:22:27Zhttps://uvadoc.uva.es/oai/requestoai:uvadoc.uva.es:10324/556132022-09-26T09:14:37Zcom_10324_40357com_10324_952com_10324_894col_10324_40358
Olive pomace phenolic compounds and extracts can inhibit inflammatory- and oxidative-related diseases of human ocular surface epithelium
Katsinas, Nicolaos
Rodríguez Rojo, Soraya
Enríquez de Salamanca y Aladro, Amalia
Cornea - Diseases
Conjunctiva - Diseases
Ojo - Enfermedades y defectos - Tratamiento
Ojo seco
Aceite de oliva
Aceite de oliva - Análisis
Antioxidantes
Oxidative- and inflammatory-related ocular surface diseases have high prevalence and are an emerging issue in ophthalmology. Olive pomace (OP) is the olive oil’s industry main by-product, and is potentially environmentally hazardous. Nevertheless, it contains phenolic compounds with important bioactivities, like oleuropein (OL) and hydroxytyrosol (HT). The antioxidant and anti-inflammatory effects of four OP extracts (CONV, OPT(1–3)), pure OL and HT, and mixtures thereof were screened on human corneal (HCE) and conjunctival epithelial (IM-ConjEpi) cells. CONV was conventionally extracted, while OPT(1–3) were produced by pressurized liquid extraction. Thanks to their improved activity, CONV and OPT3 (HT-enriched) were selected for dose-dependent studies. Cells were stimulated with tumor necrosis factor-α or ultraviolet-B radiation, measuring interleukin (IL)-1β, IL-6, IL-8, and IL-17A as well as interferon γ-induced protein [IP]-10 secretion or intracellular ROS production, respectively. On HCE, both extracts and HT inhibited the secretion of most measured ILs, demonstrating a strong anti-inflammatory effect; while in IM-ConjEpi, all samples decreased IP-10 secretion. Moreover, HT, OL, and both extracts showed strong dose-dependent antioxidant activity in both cell lines. Compared with CONV, OPT3 was active at lower concentrations, demonstrating that intensified extraction techniques are selective towards targeted biomarkers. Hence, a high-value application as potential ocular surface therapy was proposed for the OP valorization.
2022-09-23T11:17:35Z
2022-09-23T11:17:35Z
2022-09-23T11:17:35Z
2021
info:eu-repo/semantics/article
Antioxidants, 2021, Vol. 10, Nº. 7, 25 pp.
2076-3921
https://uvadoc.uva.es/handle/10324/55613
10.3390/antiox10071150
1
7
25
Antioxidants
10
2076-3921
eng
https://www.mdpi.com/2076-3921/10/7/1150
info:eu-repo/grantAgreement/EC/H2020/765608
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
© 2021 The Authors
Atribución 4.0 Internacional
MDPI