2024-03-28T21:18:30Zhttps://uvadoc.uva.es/oai/requestoai:uvadoc.uva.es:10324/224682021-09-14T08:45:36Zcom_10324_1181com_10324_931com_10324_894col_10324_1387
Differential immunomodulatory effects of Lactobacillus rhamnosus DR20, Lactobacillus fermentum CECT 5716 and Bifidobacterium animalis subsp. lactis on monocyte-derived dentritic cells
Martínez Abad, Beatriz
Garrote Adrados, José Antonio
Bernardo Ordiz, David
Montalvillo Álvarez, Enrique
Escudero Hernández, Celia
Vázquez, Enrique
Rueda, Enrique
Arranz Sanz, Eduardo
Enfermedad celíaca - Diagnóstico
Producción Científica
In this study we have identified, for the first time to our knowledge plasma CD-specific IgA Abs that recognize as specific antigen a novel deamidated gliadin peptide (FPLQPEQP), derived from an 8-mer peptide generated during duodenal degradation of gliadin by CD-specific duodenal proteases. The native peptide (FPLQPQQP) remained restricted to prolamin and glutenin proteins from toxic cereals to CD patients and was particularly expressed in ω-prolamins. The 8-mer peptide overlapped with a previously identified gluten T cell epitope and its ability to develop a humoral immune response in vivo in CD patients was confirmed by the detection of IgA anti-DGP 8-mer Abs in plasma samples from those patients.
The DGP 8-mer could be used as a novel specific CD antigen for the diagnosis of CD and monitoring of the compliance of GFD. We have designed an ELISA test which detects IgA anti-DGP 8-mer Abs in plasma samples from CD- patients with a high specificity (94% in children and 98.8% in adults) and sensitivity (93.5% in children and 81.3% in adults). Future studies with larger number of samples and additional relevant data (i.e. use of novel tools for GFD compliance) will assess the full potential of the novel diagnostic tool for celiac diagnosis and management.
2017-03-07T16:15:34Z
2017-03-07T16:15:34Z
2013
info:eu-repo/semantics/article
Plos ONE, November 2013, vol.8, n.11. p.11-12
http://uvadoc.uva.es/handle/10324/22468
1
11
12
PLOS
8
eng
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
Attribution-NonCommercial-NoDerivatives 4.0 International
Public Library of Science
SI