2024-03-28T17:49:00Zhttps://uvadoc.uva.es/oai/requestoai:uvadoc.uva.es:10324/380232021-06-24T07:49:11Zcom_10324_35294com_10324_952com_10324_894com_10324_1173com_10324_931col_10324_35295col_10324_1371
The effect of temperature during culture enrichment on methanotrophic polyhydroxyalkanoate production
Pérez Fernández, Rebeca
Cantera Ruiz de Pellón, Sara
Bordel Velasco, Sergio
García Encina, Pedro Antonio
Muñoz Torre, Raúl
Producción Científica
Climate change and plastic pollution are likely the most relevant environmental problems of the 21st Century. Thus, one of the most promising solutions to remedy both environmental problems simultaneously is the bioconversion of greenhouse gases, such as methane (CH4), into bioplastics (PHAs). However, the optimization of this bioconversion platform is still required to turn CH4 biotransformation into a cost-effective and cost-competitive process. In this context, the research presented here aimed at elucidating the best temperature culture conditions to enhance both PHA accumulation and methane degradation. Six different enrichments were carried out at 25, 30 and 37 °C using different inocula and methane as the only energy and carbon source. CH4 biodegradation rates, specific growth rates, PHA accumulations and the community structure were characterized. Higher temperatures (30 and 37 °C) increased the PHAs accumulation up to 30% regardless of the inoculum. Moreover, Methylocystis became the dominant genus (∼30% of the total population) regardless of the temperature and inoculum used. This research demonstrated for the first time the fundamental role of temperature in increasing both the accumulation of PHAs and methane abatement during the enrichment of PHA cell-factories from methane, thus enhancing the cost-effectiveness of the process.
2019-09-19T11:52:44Z
2019-09-19T11:52:44Z
2019
info:eu-repo/semantics/article
International Biodeterioration & Biodegradation Volume 140, 2019, Pages 144-151
0964-8305
http://uvadoc.uva.es/handle/10324/38023
10.1016/j.ibiod.2019.04.004
144
151
International Biodeterioration & Biodegradation
140
eng
https://www.sciencedirect.com/science/article/pii/S0964830519301520?via%3Dihub
info:eu-repo/grantAgreement/EC/H2020/750126
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
© 2019 Elsevier
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Elsevier
SI