2024-03-28T22:30:43Zhttps://uvadoc.uva.es/oai/requestoai:uvadoc.uva.es:10324/409432021-06-24T07:47:58Zcom_10324_31661com_10324_952com_10324_894com_10324_1186com_10324_931col_10324_31662col_10324_1404
Biosensors Platform Based on Chitosan/AuNPs/Phthalocyanine Composite Films for the Electrochemical Detection of Catechol. The Role of the Surface Structure
Salvo Comino, Coral
González Gil, Alfonso
Rodríguez Valentín, Javier
García Hernández, Celia
Martín Pedrosa, Fernando
García Cabezón, Ana Cristina
Rodríguez Méndez, María Luz
Producción Científica
Biosensor platforms consisting of layer by layer films combining materials with different functionalities have been developed and used to obtain improved catechol biosensors. Tyrosinase (Tyr) or laccase (Lac) were deposited onto LbL films formed by layers of a cationic linker (chitosan, CHI) alternating with layers of anionic electrocatalytic materials (sulfonated copper phthalocyanine, CuPcS or gold nanoparticles, AuNP). Films with different layer structures were successfully formed. Characterization of surface roughness and porosity was carried out using AFM. Electrochemical responses towards catechol showed that the LbL composites efficiently improved the electron transfer path between Tyr or Lac and the electrode surface, producing an increase in the intensity over the response in the absence of the LbL platform. LbL structures with higher roughness and pore size facilitated the diffusion of catechol, resulting in lower LODs. The [(CHI)-(AuNP)-(CHI)-(CuPcS)]2-Tyr showed an LOD of 8.55∙10−4 μM, which was one order of magnitude lower than the 9.55·10−3 µM obtained with [(CHI)-(CuPcS)-(CHI)-(AuNP)]2-Tyr, and two orders of magnitude lower than the obtained with other nanostructured platforms. It can be concluded that the combination of adequate materials with complementary activity and the control of the structure of the platform is an excellent strategy to obtain biosensors with improved performances.
2020-05-28T13:11:03Z
2020-05-28T13:11:03Z
2020
info:eu-repo/semantics/article
Sensors, 2020, vol. 20, n. 7. 12 p.
1424-8220
http://uvadoc.uva.es/handle/10324/40943
10.3390/s20072152
eng
https://www.mdpi.com/1424-8220/20/7/2152
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
© 2020 MDPI
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