2024-03-29T08:57:52Zhttps://uvadoc.uva.es/oai/requestoai:uvadoc.uva.es:10324/248112021-06-24T07:27:15Zcom_10324_22821com_10324_954com_10324_894col_10324_22822
Putzu, M.
5f476b2d-3533-4f04-a342-a53443b0e939
500
González de Torre, Israel
123679cbd96b8813
500
0000-0002-7795-5150
Rodríguez Cabello, José Carlos
d05e9dc813a44ad8
500
0000-0002-3438-858X
Causa, F.
02b7549a-2caa-435a-a81e-c8758fca1408
500
Netti, P.A.
e79a4e9c-94cb-44cf-99ec-8b268dd3b8c8
500
2017-07-28T10:03:03Z
2018-08-01T23:40:44Z
2016
Biofabrication 8 ( 2016 ) 045009
http://uvadoc.uva.es/handle/10324/24811
10.1088 / 1758-5090 / 8 / 4 / 045009
Producción Científica
Coronary angioplasty is the most widely used technique for removing atherosclerotic plaques in blood vessels. The regeneration of the damaged intima layer after this treatment is still one of the major challenges in the field of cardiovascular tissue engineering. Different polymers have been used in scaffold manufacturing in order to improve tissue regeneration. Elastin-mimetic polymers are a new class of molecules that have been synthesized and used to obtain small diameter fibers with specific morphological characteristics. Elastin-like polymers produced by recombinant techniques and called elastin-like recombinamers (ELRs) are particularly promising due to their high degree of functionalization. Generally speaking, ELRs can show more complex molecular designs and a tighter control of their sequence than other chemically synthetized polymers Rodriguez Cabello et al (2009 Polymer 50 5159–69, 2011 Nanomedicine 6 111–22). For the fabrication of small diameter fibers, different ELRs were dissolved in 2,2,2-fluoroethanol (TFE). Dynamic light scattering was used to identify the transition temperature and get a deep characterization of the transition behavior of the recombinamers. In this work, we describe the use of electrospinning technique for the manufacturing of an elastic fibrous scaffold; the obtained fibers were characterized and their cytocompatibility was tested in vitro. A thorough study of the influence of voltage, flow rate and distance was carried out in order to determine the appropriate parameters to obtain fibrous mats without beads and defects. Moreover, using a rotating mandrel, we fabricated a tubular scaffold in which ELRs containing different cell adhesion sequences (mainly REDV and RGD) were collected. The stability of the scaffold was improved by using genipin as a crosslinking agent. Genipin-ELRs crosslinked scaffolds show a good stability and fiber morphology. Human umbilical vein endothelial cells were used to assess the in vitro bioactivity of the cell adhesion domains within the backbone of the ELRs.
2018-08-01
application/pdf
eng
IOP Publishing
info:eu-repo/semantics/openAccess
Angioplastia coronaria
Elastin-like-recombinamers multilayered nanofibrous scaffolds for cardiovascular applications
info:eu-repo/semantics/article
http://iopscience.iop.org/article/10.1088/1758-5090/8/4/045009/meta
info:eu-repo/grantAgreement/EC/H2020/278557
SI
ORIGINAL
Elastin-Like Scaffolds.pdf
Elastin-Like Scaffolds.pdf
application/pdf
4928893
https://uvadoc.uva.es/bitstream/10324/24811/1/Elastin-Like%20Scaffolds.pdf
8f5ab61d0d7114be54191e6027ce7c78
MD5
1
LICENSE
license.txt
license.txt
text/plain
4250
https://uvadoc.uva.es/bitstream/10324/24811/2/license.txt
909e634ba52becf192e4e9b4bcde7863
MD5
2
THUMBNAIL
Elastin-Like Scaffolds.pdf.jpg
Elastin-Like Scaffolds.pdf.jpg
IM Thumbnail
image/jpeg
1216
https://uvadoc.uva.es/bitstream/10324/24811/3/Elastin-Like%20Scaffolds.pdf.jpg
eed0c3e57c0d437317c2435469139224
MD5
3
10324/24811
oai:uvadoc.uva.es:10324/24811
2021-06-24 09:27:15.528
UVaDOC
repositorio@uva.es
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