2024-03-29T10:59:08Zhttps://uvadoc.uva.es/oai/requestoai:uvadoc.uva.es:10324/409432021-06-24T07:47:58Zcom_10324_31661com_10324_952com_10324_894com_10324_1186com_10324_931col_10324_31662col_10324_1404
Salvo Comino, Coral
024ffd2fe640e684
500
González Gil, Alfonso
694b9576-73ca-4995-ad05-42765844eb3c
500
Rodríguez Valentín, Javier
f6c693ff-52dc-4ca0-9c5c-ad577e57b002
500
García Hernández, Celia
1484bfb9fad58819
500
Martín Pedrosa, Fernando
793f7f7f2c3eae34
500
0000-0002-3110-3358
García Cabezón, Ana Cristina
5b8726b7a169d418
500
0000-0003-0524-5931
Rodríguez Méndez, María Luz
8a345132091e3f84
500
0000-0002-9760-362X
2020-05-28T13:11:03Z
2020-05-28T13:11:03Z
2020
Sensors, 2020, vol. 20, n. 7. 12 p.
1424-8220
http://uvadoc.uva.es/handle/10324/40943
10.3390/s20072152
Producción Científica
Biosensor platforms consisting of layer by layer films combining materials with different functionalities have been developed and used to obtain improved catechol biosensors. Tyrosinase (Tyr) or laccase (Lac) were deposited onto LbL films formed by layers of a cationic linker (chitosan, CHI) alternating with layers of anionic electrocatalytic materials (sulfonated copper phthalocyanine, CuPcS or gold nanoparticles, AuNP). Films with different layer structures were successfully formed. Characterization of surface roughness and porosity was carried out using AFM. Electrochemical responses towards catechol showed that the LbL composites efficiently improved the electron transfer path between Tyr or Lac and the electrode surface, producing an increase in the intensity over the response in the absence of the LbL platform. LbL structures with higher roughness and pore size facilitated the diffusion of catechol, resulting in lower LODs. The [(CHI)-(AuNP)-(CHI)-(CuPcS)]2-Tyr showed an LOD of 8.55∙10−4 μM, which was one order of magnitude lower than the 9.55·10−3 µM obtained with [(CHI)-(CuPcS)-(CHI)-(AuNP)]2-Tyr, and two orders of magnitude lower than the obtained with other nanostructured platforms. It can be concluded that the combination of adequate materials with complementary activity and the control of the structure of the platform is an excellent strategy to obtain biosensors with improved performances.
Ministerio de Ciencia, Innovación y Universidades - Fondo Europeo de Desarrollo Regional (project RTI2018-097990-B-100)
Junta de Castilla y Leon - Fondo Europeo de Desarrollo Regional (project VA275P18)
Infraestructuras Red de Castilla y León (grant UVA01)
application/pdf
eng
MDPI
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
© 2020 MDPI
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Catechol
Catecol
Gold nanoparticles
Nanopartículas de oro
Laccases
Lacasas
Tyrosinase
Tirosinasa
2307 Química Física
2303 Química Inorgánica
Biosensors Platform Based on Chitosan/AuNPs/Phthalocyanine Composite Films for the Electrochemical Detection of Catechol. The Role of the Surface Structure
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
https://www.mdpi.com/1424-8220/20/7/2152
SI
THUMBNAIL
Biosensors-platform-based-on-chytosan.pdf.jpg
Biosensors-platform-based-on-chytosan.pdf.jpg
IM Thumbnail
image/jpeg
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https://uvadoc.uva.es/bitstream/10324/40943/4/Biosensors-platform-based-on-chytosan.pdf.jpg
942b31de34863b9a3a59742046b35d56
MD5
4
LICENSE
license.txt
license.txt
text/plain
3926
https://uvadoc.uva.es/bitstream/10324/40943/3/license.txt
017313583e37a1e4d1253e1ef6c66c6c
MD5
3
CC-LICENSE
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817
https://uvadoc.uva.es/bitstream/10324/40943/2/license_rdf
975848b171c53b753f7e445c8b5bc0e1
MD5
2
ORIGINAL
Biosensors-platform-based-on-chytosan.pdf
Biosensors-platform-based-on-chytosan.pdf
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2208309
https://uvadoc.uva.es/bitstream/10324/40943/1/Biosensors-platform-based-on-chytosan.pdf
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oai:uvadoc.uva.es:10324/40943
2021-06-24 09:47:58.795
UVaDOC
repositorio@uva.es
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