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oai:uvadoc.uva.es:10324/410342021-06-23T09:52:42Zcom_10324_1134com_10324_931com_10324_894col_10324_1213

Muñoz Martínez, Raquel 30d13e8260075711 500 0000-0001-9861-0349 Klingenberg, Olav beb612d1-7ec2-4437-950d-0b5f227040da 500 Wiedlocha, Antoni 85d7dca2-2e04-438b-a48c-25383ba5d1a6 500 Rapak, Andrzej 43c19576-ba7f-479f-86db-80c29feb4951 500 Falnes, Pål 4569e194-c979-4863-8bf2-cf859a58e1d0 500 Olsnes, Sjur 55bbd5d8-e665-4d3b-81c9-c0c9eb0c2bbf 500 2020-06-12T11:20:51Z 2020-06-12T11:20:51Z 1997 Oncogene, 1997, vol. 15, n. 5. p. 525-536 1476-5594 http://uvadoc.uva.es/handle/10324/41034 10.1038/sj.onc.1201226 Producción Científica Acidic fibroblast growth factor (aFGF) binds to specific transmembrane receptors and is partly transported to a nuclear location. To study this transport we made a kinase-negative mutant of FGF receptor 4 as well as one where the major part of the cytoplasmic receptor domain was deleted, and expressed them in U2OSDr1 cells that lack functional FGF receptors. All receptors mediated endocytic uptake of aFGF. Translocation of the growth factor across cellular membranes was assayed using aFGF with a C-terminal CAAX-motif, which signals addition of a farnesyl group onto the protein once in the cytosol. CAAX-tagged aFGF was farnesylated when incubated with cells containing wild-type or kinase-negative receptors. It was not farnesylated in cells expressing the deleted receptor, or when the incubation was in the presence of genistein. aFGF incubated with cells transfected with wild-type or kinase-negative receptors, but not with the deleted receptor, was partly recovered from the nuclear fraction in the absence, but not in the presence of genistein. The data indicate that the cytoplasmic receptor domain, but not the active kinase, is required for transport of the growth factor into cells, and that genistein inhibits the process. Ministerio de Educación y Formación Profesional - Unión Europea (grant ERB4001GT954487) application/pdf eng Springer Nature info:eu-repo/semantics/openAccess © 1997 Springer Nature Receptor mutant Receptor mutante Genistein Genisteína Farnesylation Farnesilación Effect of mutation of cytoplasmic receptor domain and of genistein on transport of acidic fibroblast growth factor into cells info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.nature.com/articles/1201226 SI THUMBNAIL Effect-of-mutation-of-cytoplasmic-receptor.pdf.jpg Effect-of-mutation-of-cytoplasmic-receptor.pdf.jpg IM Thumbnail image/jpeg 1869 https://uvadoc.uva.es/bitstream/10324/41034/3/Effect-of-mutation-of-cytoplasmic-receptor.pdf.jpg 227d55e7d3bcc269ad7faa9019daa306 MD5 3 LICENSE license.txt license.txt text/plain 3926 https://uvadoc.uva.es/bitstream/10324/41034/2/license.txt 017313583e37a1e4d1253e1ef6c66c6c MD5 2 ORIGINAL Effect-of-mutation-of-cytoplasmic-receptor.pdf Effect-of-mutation-of-cytoplasmic-receptor.pdf application/pdf 636598 https://uvadoc.uva.es/bitstream/10324/41034/1/Effect-of-mutation-of-cytoplasmic-receptor.pdf fe41cf7661ed010e7a90d1ffbf826326 MD5 1 10324/41034 oai:uvadoc.uva.es:10324/41034 2021-06-23 11:52:42.563 UVaDOC repositorio@uva.es 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