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oai:uvadoc.uva.es:10324/450742021-06-24T07:17:03Zcom_10324_44585com_10324_954com_10324_894col_10324_44586

Villalobos Jorge, Carlos 84541d88-c154-4cd4-a1a9-0dbab3dda414 500 Núñez Llorente, Lucía 095f01c02739fe93 500 Faught, William J. 232025ec-fc09-4a08-8b75-7463bf1b46b2 500 Leaumont, David C. 9a9f6f32-c19c-4716-ac18-c54a3ddf4c30 500 Boockfor, Fredric R. bc3e841a-0ccd-4722-b7ba-036127526409 500 Frawley, L. Stephen 0b9e639e-b5cf-4286-a862-b784a28d8b13 500 2021-01-19T11:57:18Z 2021-01-19T11:57:18Z 2002 Endocrinology, 2002, vol. 143, n. 9. p. 3548–3554 1945-7170 http://uvadoc.uva.es/handle/10324/45074 10.1210/en.2002-220266 Producción Científica Research on the regulation of hormone gene expression by calcium signaling is hampered by the difficulty of monitoring both parameters within the same individual, living cells. Here we achieved concurrent, dynamic measurements of both intracellular Ca2+ concentration ([Ca2+]i) and prolactin (PRL) gene promoter activity in single, living pituitary cells. Cells were transfected with the luciferase reporter gene under control of the PRL promoter and subjected to bioluminescence and fluorescence imaging before and after presentation of TSH-releasing hormone (TRH), a prototypic regulator of PRL secretion and gene expression that induces a transient Ca2+ release, followed by sustained Ca2+ influx. We found that cells displaying specific photonic emissions (i.e. mammotropes) showed heterogeneous calcium and transcriptional responses to TRH. Transcriptionally responsive cells always exhibited a TRH-induced [Ca2+]i increase. In addition, transcriptional responses were related to the rate of Ca2+ entry but not Ca2+ release. Finally, cells lacking transcriptional responses (but showing [Ca2+]i rises) exhibited larger levels of resting PRL promoter activity than transcriptionally responsive cells. Thus, our results suggest that the sustained entry of Ca2+ induced by TRH (but not the Ca2+ release) regulates transcriptional responsiveness. Superimposed on this regulation, the previous, resting PRL promoter activity also controls transcriptional responses. National Institutes of Health (grant DK-38215) Fondo de Investigaciones Sanitarias (grant FIS 01/0769) application/pdf eng Oxford University Press info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/3.0/ © 2002 Oxford University Press Attribution-NonCommercial-NoDerivs 3.0 Unported Gene expression Expresión génica Prolactin Prolactina Calcium dynamics and resting transcriptional activity regulates prolactin gene expression info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://academic.oup.com/endo/article/143/9/3548/2880874 SI THUMBNAIL Calcium-dynamics-and-resting.pdf.jpg Calcium-dynamics-and-resting.pdf.jpg IM Thumbnail image/jpeg 3853 https://uvadoc.uva.es/bitstream/10324/45074/4/Calcium-dynamics-and-resting.pdf.jpg bc58adf7e740d1b4886cfecc6617dafb MD5 4 LICENSE license.txt license.txt text/plain 3926 https://uvadoc.uva.es/bitstream/10324/45074/3/license.txt 017313583e37a1e4d1253e1ef6c66c6c MD5 3 CC-LICENSE license_rdf license_rdf application/rdf+xml; charset=utf-8 817 https://uvadoc.uva.es/bitstream/10324/45074/2/license_rdf bb40ef9f1fe160072c35924bc4d018c4 MD5 2 ORIGINAL Calcium-dynamics-and-resting.pdf Calcium-dynamics-and-resting.pdf application/pdf 418128 https://uvadoc.uva.es/bitstream/10324/45074/1/Calcium-dynamics-and-resting.pdf d167f677aebad63133ac0506b59abab1 MD5 1 10324/45074 oai:uvadoc.uva.es:10324/45074 2021-06-24 09:17:03.519 UVaDOC repositorio@uva.es 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