2026-05-11T14:51:52Zhttps://uvadoc.uva.es/oai/request

oai:uvadoc.uva.es:10324/491562021-11-17T12:38:51Zcom_10324_31661com_10324_952com_10324_894col_10324_31662

Salvo Comino, Coral 024ffd2fe640e684 600 Martín Pedrosa, Fernando 793f7f7f2c3eae34 600 García Cabezón, Ana Cristina 5b8726b7a169d418 600 0000-0003-0524-5931 Rodríguez Méndez, María Luz 8a345132091e3f84 600 0000-0002-9760-362X 2021-10-19T08:01:47Z 2021-10-19T08:01:47Z 2021 Sensors, 2021, vol. 21, n. 3, 899 1424-8220 https://uvadoc.uva.es/handle/10324/49156 10.3390/s21030899 899 3 Sensors 21 1424-8220 Producción Científica The integration of nanomaterials as electron mediators in electrochemical biosensors is taking on an essential role. Due to their high surface-to-volume ratio and high conductivity, metallic nanowires are an interesting option. In this paper, silver nanowires (AgNWs) were exploited to design a novel catechol electrochemical biosensor, and the benefits of increasing the aspect ratio of the electron mediator (nanowires vs. nanoparticles) were analyzed. Atomic force microscopy (AFM) studies have shown a homogeneous distribution of the enzyme along the silver nanowires, maximizing the contact surface. The large contact area promotes electron transfer between the enzyme and the electrode surface, resulting in a Limit of Detection (LOD) of 2.7 × 10−6 M for tyrosinase immobilized onto AgNWs (AgNWs-Tyr), which is one order of magnitude lower than the LOD of 3.2 × 10−5 M) obtained using tyrosinase immobilized onto silver nanoparticles (AgNPs-Tyr). The calculated KM constant was 122 mM. The simultaneous use of electrochemistry and AFM has demonstrated a limited electrochemical fouling that facilitates stable and reproducible detection. Finally, the biosensor showed excellent anti-interference characteristics toward the main phenols present in wines including vanillin, pyrogallol, quercetin and catechin. The biosensor was able to successfully detect the presence of catechol in real wine samples. These results make AgNWs promising elements in nanowired biosensors for the sensitive, stable and rapid voltammetric detection of phenols in real applications. Ministerio de Ciencia Innovación y Universidades - FEDER (project RTI2018-097990-B-100) Junta de Castilla y León- FEDER (project VA275P18) e (Infraestructuras Red de Castilla y León (INFRARED) UVA01) application/pdf eng MDPI info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/ © 2021 MDPI Atribución 4.0 Internacional Silver nanowires Tyrosinase Electrochemical biosensor Catechol 23 Química 22 Física Silver nanowires as electron transfer mediators in electrochemical catechol biosensors info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://www.mdpi.com/1424-8220/21/3/899/htm SI THUMBNAIL Silver-nanowires-as-electron.pdf.jpg Silver-nanowires-as-electron.pdf.jpg IM Thumbnail image/jpeg 3449 https://uvadoc.uva.es/bitstream/10324/49156/4/Silver-nanowires-as-electron.pdf.jpg a8d5bf769fe2217ee24a43d5c919184a MD5 4 LICENSE license.txt license.txt text/plain 3926 https://uvadoc.uva.es/bitstream/10324/49156/3/license.txt 017313583e37a1e4d1253e1ef6c66c6c MD5 3 CC-LICENSE license_rdf license_rdf application/rdf+xml; charset=utf-8 921 https://uvadoc.uva.es/bitstream/10324/49156/2/license_rdf 9eecc1514e7247cad41e4db756248760 MD5 2 ORIGINAL Silver-nanowires-as-electron.pdf Silver-nanowires-as-electron.pdf application/pdf 1856110 https://uvadoc.uva.es/bitstream/10324/49156/1/Silver-nanowires-as-electron.pdf 12e125be8ce5f910d1c2e547e66d2248 MD5 1 10324/49156 oai:uvadoc.uva.es:10324/49156 2021-11-17 13:38:51.676 UVaDOC repositorio@uva.es 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