2024-03-29T11:39:37Zhttps://uvadoc.uva.es/oai/requestoai:uvadoc.uva.es:10324/59702021-06-23T09:48:21Zcom_10324_1133com_10324_931com_10324_894col_10324_1209
Montero Zoccola, María Teresa
0ea84f9ae27b5cd1
500
0000-0001-7702-6653
Alonso Alonso, María Teresa
fa2994068281858f
500
Carnicero Gila, Estela María
8bad004b0346786b
500
0000-0002-9236-5503
Cuchillo Ibáñez, Inmaculada
60d69bfc-ab33-4351-a09f-520c358dec39
500
Albillos, Almudena
f6f6da83-ad23-4585-8109-838f9d98ebfd
500
García, Antonio G.
57f33cbe-6809-402a-b718-c5b537f4a80f
500
García-Sancho Martín, Francisco Javier
6c6cfe3c63dd2c44
500
0000-0003-4573-7930
Álvarez Martín, Javier
cff9feef39aa55ff
500
0000-0003-0636-5521
2014-09-16T08:08:18Z
2015-03-16T00:40:07Z
2000
Nature Cell Biology, Febrero 2000, vol. 2, p. 57-61
1465-7392
http://uvadoc.uva.es/handle/10324/5970
57
61
Nature Cell Biology
2
Producción Científica
Activation of calcium-ion (Ca2+) channels on the plasma membrane and on intracellular Ca2+ stores, such as the
endoplasmic reticulum, generates local transient increases in the cytosolic Ca2+ concentration that induce Ca2+ uptake
by neighbouring mitochondria. Here, by using mitochondrially targeted aequorin proteins with different Ca2+ affinities,
we show that half of the chromaffin-cell mitochondria exhibit surprisingly rapid millimolar Ca2+ transients upon
stimulation of cells with acetylcholine, caffeine or high concentrations of potassium ions. Our results show a tight
functional coupling of voltage-dependent Ca2+ channels on the plasma membrane, ryanodine receptors on the
endoplasmic reticulum, and mitochondria. Cell stimulation generates localized Ca2+ transients, with Ca2+ concentrations
above 20–40 mM, at these functional units. Protonophores abolish mitochondrial Ca2+ uptake and increase stimulated
secretion of catecholamines by three- to fivefold. These results indicate that mitochondria modulate secretion by
controlling the availability of Ca2+ for exocytosis.
2015-03-16
application/pdf
eng
Macmillan Magazines Ltd.
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
Attribution-NonCommercial-NoDerivatives 4.0 International
Calcio - Efectos fisiológicos
Chromaffin-cell stimulation triggers fast millimolar mitochondrial Ca2+ transients that modulate secretion
info:eu-repo/semantics/article
SI
CC-LICENSE
license_url
license_url
text/plain
49
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ORIGINAL
ALVAREZ 12- Chromaffin-cell stimulation.pdf
ALVAREZ 12- Chromaffin-cell stimulation.pdf
PD-107
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469155
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10324/5970
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2021-06-23 11:48:21.357
UVaDOC
repositorio@uva.es
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